Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

Xiao Chen Bai, Israel S. Fernandez, Greg McMullan, Sjors H W Scheres

Research output: Contribution to journalArticle

281 Scopus citations

Abstract

Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of large and flexible macro-molecular assemblies, the technique has not yet reached its full potential. Besides fundamental limits imposed by radiation damage, poor detectors and beam-induced sample movement have been shown to degrade attainable resolutions. A new generation of direct electron detectors may ameliorate both effects. Apart from exhibiting improved signal-to-noise performance, these cameras are also fast enough to follow particle movements during electron irradiation. Here, we assess the potentials of this technology for cryo-EM structure determination. Using a newly developed statistical movie processing approach to compensate for beam-induced movement, we show that ribosome reconstructions with unprecedented resolutions may be calculated from almost two orders of magnitude fewer particles than used previously. Therefore, this methodology may expand the scope of high-resolution cryo-EM to a broad range of biological specimens.

Original languageEnglish (US)
Article numbere00461
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Feb 19 2013

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles'. Together they form a unique fingerprint.

  • Cite this