Ring-like nucleoid does not play a key role in radioresistance of Deinococcus radiodurans

GuanJun Gao, HuiMing Lu, LongFei Yin, YueJin Hua

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The conclusion based on transmission electron microscopy, "the tightly packed ring-like nucleoid of the Deinococcus radiodurans R1 is a key to radioresistance", has instigated lots of debates. In this study, according to the previous research of Pprl's crucial role in radioresistance of D. radiodurans, we have attempted to examine and compare the nucleoid morphology differences among wild-type D. radiodurans R1 strain, pprf function-deficient mutant (YR1), and pprl function-complementary strains (YR1001, YR1002, and YR1004) before and after exposure to ionizing irradiation. Fluorescence microscopy images indicate: (1) the majority of nucleoid structures in radioresistant strain R1 cells exhibit the tightly packed ring-like morphology, while the pprl function-deficient mutant YR1 cells carrying predominate ring-like structure represent high sensitivity to irradiation; (2) as an extreme radioresistant strain similar to wild-type R1, pprl completely function-complementary strain YR1001 almost displays the loose and irregular nucleoid morphologies. On the other hand, another radioresistant pprl partly function-complementary strain YR1002's nucleiods exhibit about 60% ring-like structure; (3) a Pprl C-terminal deletion strain YR1004 consisting of approximately 60% of ring-like nucleoid is very sensitive to radiation. Therefore, our present experiments do not support the conclusion that the ring-like nucleoid of D. radiodurans does play a key role in radioresistance.

Original languageEnglish (US)
Pages (from-to)525-529
Number of pages5
JournalScience in China, Series C: Life Sciences
Volume50
Issue number4
DOIs
StatePublished - Aug 1 2007

Fingerprint

Deinococcus
Deinococcus radiodurans
radiation resistance
irradiation
transmission electron microscopy
microscopy
fluorescence
Transmission Electron Microscopy
Fluorescence Microscopy
Irradiation
mutants
Radiation
Fluorescence microscopy
fluorescence microscopy
ionization
experiment
Research
cells
mutant
Transmission electron microscopy

Keywords

  • Deinococcus radiodurans R1
  • Ionizing irradiation
  • pprl
  • Radioresistance
  • Ring-like nucleoid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Ring-like nucleoid does not play a key role in radioresistance of Deinococcus radiodurans. / Gao, GuanJun; Lu, HuiMing; Yin, LongFei; Hua, YueJin.

In: Science in China, Series C: Life Sciences, Vol. 50, No. 4, 01.08.2007, p. 525-529.

Research output: Contribution to journalArticle

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abstract = "The conclusion based on transmission electron microscopy, {"}the tightly packed ring-like nucleoid of the Deinococcus radiodurans R1 is a key to radioresistance{"}, has instigated lots of debates. In this study, according to the previous research of Pprl's crucial role in radioresistance of D. radiodurans, we have attempted to examine and compare the nucleoid morphology differences among wild-type D. radiodurans R1 strain, pprf function-deficient mutant (YR1), and pprl function-complementary strains (YR1001, YR1002, and YR1004) before and after exposure to ionizing irradiation. Fluorescence microscopy images indicate: (1) the majority of nucleoid structures in radioresistant strain R1 cells exhibit the tightly packed ring-like morphology, while the pprl function-deficient mutant YR1 cells carrying predominate ring-like structure represent high sensitivity to irradiation; (2) as an extreme radioresistant strain similar to wild-type R1, pprl completely function-complementary strain YR1001 almost displays the loose and irregular nucleoid morphologies. On the other hand, another radioresistant pprl partly function-complementary strain YR1002's nucleiods exhibit about 60{\%} ring-like structure; (3) a Pprl C-terminal deletion strain YR1004 consisting of approximately 60{\%} of ring-like nucleoid is very sensitive to radiation. Therefore, our present experiments do not support the conclusion that the ring-like nucleoid of D. radiodurans does play a key role in radioresistance.",
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AB - The conclusion based on transmission electron microscopy, "the tightly packed ring-like nucleoid of the Deinococcus radiodurans R1 is a key to radioresistance", has instigated lots of debates. In this study, according to the previous research of Pprl's crucial role in radioresistance of D. radiodurans, we have attempted to examine and compare the nucleoid morphology differences among wild-type D. radiodurans R1 strain, pprf function-deficient mutant (YR1), and pprl function-complementary strains (YR1001, YR1002, and YR1004) before and after exposure to ionizing irradiation. Fluorescence microscopy images indicate: (1) the majority of nucleoid structures in radioresistant strain R1 cells exhibit the tightly packed ring-like morphology, while the pprl function-deficient mutant YR1 cells carrying predominate ring-like structure represent high sensitivity to irradiation; (2) as an extreme radioresistant strain similar to wild-type R1, pprl completely function-complementary strain YR1001 almost displays the loose and irregular nucleoid morphologies. On the other hand, another radioresistant pprl partly function-complementary strain YR1002's nucleiods exhibit about 60% ring-like structure; (3) a Pprl C-terminal deletion strain YR1004 consisting of approximately 60% of ring-like nucleoid is very sensitive to radiation. Therefore, our present experiments do not support the conclusion that the ring-like nucleoid of D. radiodurans does play a key role in radioresistance.

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