Rmrp is a non-coding rna essential for early murine development

Joseph Rosenbluh, Deepak Nijhawan, Zhao Chen, Kwok Kin Wong, Kenkichi Masutomi, William C. Hahn

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27 Scopus citations

Abstract

RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development.

Original languageEnglish (US)
Article numbere26270
JournalPloS one
Volume6
Issue number10
DOIs
StatePublished - Oct 25 2011

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Rosenbluh, J., Nijhawan, D., Chen, Z., Wong, K. K., Masutomi, K., & Hahn, W. C. (2011). Rmrp is a non-coding rna essential for early murine development. PloS one, 6(10), [e26270]. https://doi.org/10.1371/journal.pone.0026270