RNA polymerase II transcription factor SIII. I. Identification, purification, and properties

J. N. Bradsher, K. W. Jackson, R. C. Conaway, J. W. Conaway

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

A transcription factor that stimulates synthesis of accurately initiated transcripts by RNA polymerase II has been identified and purified to apparent homogeneity from rat liver nuclear extracts. This factor, which we designate SIII, has a native molecular mass of ~140 kDa and is composed of three polypeptides of 110, 18, and 15 kDa. All three polypeptides are required to reconstitute SIII transcriptional activity. SIII appears distinct from previously identified mammalian transcription factors.

Original languageEnglish (US)
Pages (from-to)25587-25593
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number34
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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