RNA polymerase II transcription: Structure and mechanism

Xin Liu, David A. Bushnell, Roger D. Kornberg

Research output: Contribution to journalReview articlepeer-review

68 Scopus citations

Abstract

A minimal RNA polymerase II (pol II) transcription system comprises the polymerase and five general transcription factors (GTFs) TFIIB, -D, -E, -F, and -H. The addition of Mediator enables a response to regulatory factors. The GTFs are required for promoter recognition and the initiation of transcription. Following initiation, pol II alone is capable of RNA transcript elongation and of proofreading. Structural studies reviewed here reveal roles of GTFs in the initiation process and shed light on the transcription elongation mechanism. This article is part of a Special Issue entitled: RNA Polymerase II Transcript Elongation.

Original languageEnglish (US)
Pages (from-to)2-8
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1829
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Backtracked state
  • Bridge helix
  • Hybrid helix melting
  • TFIIB
  • Trigger loop

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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