Abstract
Detailed characterization of cellular RNA facilitates the design of nucleic acid therapeutics and interpretation of experimental data. Since the first reports of next generation sequencing (NGS) technology-based RNA sequencing (RNA-seq) (Nagalakshmi et al., 2008; Wilhelm, et al., 2008), rapid advances in experimental protocol development and data acquisition and analysis have brought comprehensive sequencing of cellular RNA within reach of most laboratories (Martin and Wang, 2011; Ozsolak and Milos, 2011). While RNA-seq is becoming widely used for laboratory research and clinical studies, the field is still new and strategies for successfully applying RNA-seq to a given experimental problem are often obscure. The goal of this perspective is to introduce some of the choices confronted during RNA sequencing and analysis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 271-274 |
| Number of pages | 4 |
| Journal | Nucleic Acid Therapeutics |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2012 |
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ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Molecular Medicine
- Biochemistry
- Drug Discovery
Cite this
RNA sequencing : Platform selection, experimental design, and data interpretation. / Chu, Yongjun; Corey, David R.
In: Nucleic Acid Therapeutics, Vol. 22, No. 4, 01.08.2012, p. 271-274.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RNA sequencing
T2 - Platform selection, experimental design, and data interpretation
AU - Chu, Yongjun
AU - Corey, David R.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - Detailed characterization of cellular RNA facilitates the design of nucleic acid therapeutics and interpretation of experimental data. Since the first reports of next generation sequencing (NGS) technology-based RNA sequencing (RNA-seq) (Nagalakshmi et al., 2008; Wilhelm, et al., 2008), rapid advances in experimental protocol development and data acquisition and analysis have brought comprehensive sequencing of cellular RNA within reach of most laboratories (Martin and Wang, 2011; Ozsolak and Milos, 2011). While RNA-seq is becoming widely used for laboratory research and clinical studies, the field is still new and strategies for successfully applying RNA-seq to a given experimental problem are often obscure. The goal of this perspective is to introduce some of the choices confronted during RNA sequencing and analysis.
AB - Detailed characterization of cellular RNA facilitates the design of nucleic acid therapeutics and interpretation of experimental data. Since the first reports of next generation sequencing (NGS) technology-based RNA sequencing (RNA-seq) (Nagalakshmi et al., 2008; Wilhelm, et al., 2008), rapid advances in experimental protocol development and data acquisition and analysis have brought comprehensive sequencing of cellular RNA within reach of most laboratories (Martin and Wang, 2011; Ozsolak and Milos, 2011). While RNA-seq is becoming widely used for laboratory research and clinical studies, the field is still new and strategies for successfully applying RNA-seq to a given experimental problem are often obscure. The goal of this perspective is to introduce some of the choices confronted during RNA sequencing and analysis.
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U2 - 10.1089/nat.2012.0367
DO - 10.1089/nat.2012.0367
M3 - Article
VL - 22
SP - 271
EP - 274
JO - Nucleic Acid Therapeutics
JF - Nucleic Acid Therapeutics
SN - 2159-3337
IS - 4
ER -