RNA sequencing: Platform selection, experimental design, and data interpretation

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Detailed characterization of cellular RNA facilitates the design of nucleic acid therapeutics and interpretation of experimental data. Since the first reports of next generation sequencing (NGS) technology-based RNA sequencing (RNA-seq) (Nagalakshmi et al., 2008; Wilhelm, et al., 2008), rapid advances in experimental protocol development and data acquisition and analysis have brought comprehensive sequencing of cellular RNA within reach of most laboratories (Martin and Wang, 2011; Ozsolak and Milos, 2011). While RNA-seq is becoming widely used for laboratory research and clinical studies, the field is still new and strategies for successfully applying RNA-seq to a given experimental problem are often obscure. The goal of this perspective is to introduce some of the choices confronted during RNA sequencing and analysis.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalNucleic Acid Therapeutics
Volume22
Issue number4
DOIs
StatePublished - Aug 1 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery

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