Robust colorimetric assays for dynamin's basal and stimulated GTPase activities

Marilyn Leonard, Byeong Doo Song, Rajesh Ramachandran, Sandra L. Schmid

Research output: Contribution to journalReview article

66 Scopus citations

Abstract

Dynamin, unlike many GTPase superfamily members, exhibits a relatively rapid basal rate of GTP hydrolysis that is not rate-limited by GTP binding or GDP dissociation. Also unique to dynamin GTPase family members is their ability to self-assemble into rings and helical stacks of rings either in solution or onto lipid templates. Self-assembly stimulates dynamin's GTPase activity by >100-fold. Given these robust rates of GTP hydrolysis compared to most GTPases, GTP hydrolysis by dynamin can be easily measured using a simple colorimetic assay to detect released phosphate. We describe this assay and report variations in assay conditions that have contributed to the wide range of reported values for dynamin's basal and assembly-stimulated rates of GTP hydrolysis.

Original languageEnglish (US)
Article number43
Pages (from-to)490-503
Number of pages14
JournalMethods in Enzymology
Volume404
StatePublished - Jan 1 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Leonard, M., Doo Song, B., Ramachandran, R., & Schmid, S. L. (2005). Robust colorimetric assays for dynamin's basal and stimulated GTPase activities. Methods in Enzymology, 404, 490-503. [43].