Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

Toshiki Itoh, Tadahiro Shiomi, Naoko Shiomi, Yoshinobu Harada, Mitsuo Wakasugi, Tsukasa Matsunaga, Osamu Nikaido, Errol C. Friedberg, Masaru Yamaizumi

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalMutation Research - DNA Repair
Volume362
Issue number2
DOIs
StatePublished - Feb 15 1996

Keywords

  • (6-4) photoproduct
  • Cockayne syndrome
  • Complementation test
  • Thymine dimer
  • Xeroderma pigmentosum

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

Fingerprint Dive into the research topics of 'Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A'. Together they form a unique fingerprint.

Cite this