Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A

Toshiki Itoh, Tadahiro Shiomi, Naoko Shiomi, Yoshinobu Harada, Mitsuo Wakasugi, Tsukasa Matsunaga, Osamu Nikaido, Errol C. Friedberg, Masaru Yamaizumi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalMutation Research - DNA Repair
Volume362
Issue number2
DOIs
StatePublished - Feb 15 1996

Fingerprint

Cockayne Syndrome
Pyrimidine Dimers
Rodentia
Fusion reactions
Cell Fusion
Genetic Complementation Test
Cells
Irradiation
Kinetics
T-cells
Cell Line
Cloning
Transfection
Genes
Monoclonal Antibodies
Organism Cloning
T-Lymphocytes

Keywords

  • (6-4) photoproduct
  • Cockayne syndrome
  • Complementation test
  • Thymine dimer
  • Xeroderma pigmentosum

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Toxicology

Cite this

Itoh, T., Shiomi, T., Shiomi, N., Harada, Y., Wakasugi, M., Matsunaga, T., ... Yamaizumi, M. (1996). Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A. Mutation Research - DNA Repair, 362(2), 167-174. https://doi.org/10.1016/0921-8777(95)00046-1

Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A. / Itoh, Toshiki; Shiomi, Tadahiro; Shiomi, Naoko; Harada, Yoshinobu; Wakasugi, Mitsuo; Matsunaga, Tsukasa; Nikaido, Osamu; Friedberg, Errol C.; Yamaizumi, Masaru.

In: Mutation Research - DNA Repair, Vol. 362, No. 2, 15.02.1996, p. 167-174.

Research output: Contribution to journalArticle

Itoh, T, Shiomi, T, Shiomi, N, Harada, Y, Wakasugi, M, Matsunaga, T, Nikaido, O, Friedberg, EC & Yamaizumi, M 1996, 'Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A', Mutation Research - DNA Repair, vol. 362, no. 2, pp. 167-174. https://doi.org/10.1016/0921-8777(95)00046-1
Itoh, Toshiki ; Shiomi, Tadahiro ; Shiomi, Naoko ; Harada, Yoshinobu ; Wakasugi, Mitsuo ; Matsunaga, Tsukasa ; Nikaido, Osamu ; Friedberg, Errol C. ; Yamaizumi, Masaru. / Rodent complementation group 8 ( ERCC8) corresponds to Cockayne syndrome complementation group A. In: Mutation Research - DNA Repair. 1996 ; Vol. 362, No. 2. pp. 167-174.
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