Role of A and B blood group, antigens in the expression of adhesive activity of von Willebrand factor

Ravindra Sarode, Jack Goldstein, Ira I. Sussman, Ronald L. Nagel, Han Mou Tsai

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

ABO (H) blood group antigens are covalently linked to the oligosaccharide side-chains of yon Willebrand factor (VWF). In this study, we investigated the role of the A and B antigens in the expression of VWF adhesive activity. VWF of type A, B or O was purified from fresh frozen plasma. Presence of A or B antigen on the VWF was confirmed by enzyme-linked immunosorbent assay (ELISA) and by immunoblotting with monoclonal anti-A or anti-B. The A or B antigen was also detected in the 48/52-kDa fragment of the respective VWF after trypsin digestion. Removal of A antigen with α-N-acetylgalactosminidase or B antigen with α- galactosidase did not affect its multimer size or antigenic level, but decreased the ristocetin cofactor (RCoF) activity of the respective VWF by 33-39% (P < 0·01-0·002). Removal of A or B antigen from VWF did not affect the binding of the VWF to immobilized type III collagen. A and B antigens were not detected in platelet VWE. These results indicate that AB structures play a role in platelet aggregating activity of VWF.

Original languageEnglish (US)
Pages (from-to)857-864
Number of pages8
JournalBritish Journal of Haematology
Volume109
Issue number4
DOIs
Publication statusPublished - 2000

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Keywords

  • ABO blood group
  • Bleeding disorder
  • Platelet
  • Ristocetin cofactor activity
  • Thrombosis
  • Von Willebrand factor

ASJC Scopus subject areas

  • Hematology

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