Role of A and B blood group, antigens in the expression of adhesive activity of von Willebrand factor

Ravindra Sarode, Jack Goldstein, Ira I. Sussman, Ronald L. Nagel, Han Mou Tsai

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

ABO (H) blood group antigens are covalently linked to the oligosaccharide side-chains of yon Willebrand factor (VWF). In this study, we investigated the role of the A and B antigens in the expression of VWF adhesive activity. VWF of type A, B or O was purified from fresh frozen plasma. Presence of A or B antigen on the VWF was confirmed by enzyme-linked immunosorbent assay (ELISA) and by immunoblotting with monoclonal anti-A or anti-B. The A or B antigen was also detected in the 48/52-kDa fragment of the respective VWF after trypsin digestion. Removal of A antigen with α-N-acetylgalactosminidase or B antigen with α- galactosidase did not affect its multimer size or antigenic level, but decreased the ristocetin cofactor (RCoF) activity of the respective VWF by 33-39% (P < 0·01-0·002). Removal of A or B antigen from VWF did not affect the binding of the VWF to immobilized type III collagen. A and B antigens were not detected in platelet VWE. These results indicate that AB structures play a role in platelet aggregating activity of VWF.

Original languageEnglish (US)
Pages (from-to)857-864
Number of pages8
JournalBritish Journal of Haematology
Volume109
Issue number4
DOIs
StatePublished - 2000

Fingerprint

von Willebrand Factor
Blood Group Antigens
Adhesives
Antigens
Blood Platelets
Galactosidases
ABO Blood-Group System
Collagen Type III
Oligosaccharides
Immunoblotting
Trypsin
Digestion
Enzyme-Linked Immunosorbent Assay

Keywords

  • ABO blood group
  • Bleeding disorder
  • Platelet
  • Ristocetin cofactor activity
  • Thrombosis
  • Von Willebrand factor

ASJC Scopus subject areas

  • Hematology

Cite this

Role of A and B blood group, antigens in the expression of adhesive activity of von Willebrand factor. / Sarode, Ravindra; Goldstein, Jack; Sussman, Ira I.; Nagel, Ronald L.; Tsai, Han Mou.

In: British Journal of Haematology, Vol. 109, No. 4, 2000, p. 857-864.

Research output: Contribution to journalArticle

Sarode, Ravindra ; Goldstein, Jack ; Sussman, Ira I. ; Nagel, Ronald L. ; Tsai, Han Mou. / Role of A and B blood group, antigens in the expression of adhesive activity of von Willebrand factor. In: British Journal of Haematology. 2000 ; Vol. 109, No. 4. pp. 857-864.
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