Role of antibody and complement in the control of Encephalitozoon cuniculi infections by rabbit macrophages

J. Y. Niederkorn, J. A. Shadduck

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The capacity of mononuclear peritoneal macrophages to phagocytose Encephalitozoon cuniculi was tested in vitro. Normal rabbit serum or cell culture medium had little effect on the rate of removal of organisms by rabbit peritoneal macrophages. Treatment with immune rabbit serum or immune rabbit immunoglobulin G significantly (P < 0.001) increased phagocytosis of E. cuniculi. Guinea pig complement was found to significantly (P < 0.001) enhance the phagocytosis of antibody-treated E. cuniculi. With few exceptions, induced (peritoneal exudate) macrophages were no more effective than unstimulated (resident) macrophages in the phagocytosis of E. cuniculi. Secondary lysosomes labeled with ferritin were seen fusing with phagosomes containing immune rabbit serum-treated parasites. Phagosome-lysosome fusion was not observed when parasites were treated with either normal rabbit serum or culture medium. The results of the present study suggest a role for antibody enhancement of phagocytosis and intracellular killing as a mechanism of resistance to encephalitozoonosis in rabbits.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalInfection and Immunity
Volume27
Issue number3
StatePublished - 1980

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Encephalitozoon cuniculi
Macrophages
Phagocytosis
Rabbits
Antibodies
Infection
Peritoneal Macrophages
Phagosomes
Lysosomes
Encephalitozoonosis
Culture Media
Immune Sera
Parasites
Exudates and Transudates
Ferritins
Serum
Guinea Pigs
Cell Culture Techniques
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology

Cite this

Role of antibody and complement in the control of Encephalitozoon cuniculi infections by rabbit macrophages. / Niederkorn, J. Y.; Shadduck, J. A.

In: Infection and Immunity, Vol. 27, No. 3, 1980, p. 995-1002.

Research output: Contribution to journalArticle

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