Role of carboxylmethylation in chemoattractant receptor-stimulated G protein activation and functional responses

Eleanor D. Lederer, Alfred A. Jacobs, Jerald L. Hoffman, George B. Harding, Janet D. Robishaw, Kenneth R. McLeish

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The role of G protein γ subunit carboxylmethylation was examined in HL-60 granulocytes using an inhibitor of S-adenosylmethionine-dependent methylation, periodate-oxidized adenosine (Adox). A 40-60% reduction in γ subunit carboxylmethylation was associated with attenuation of fMet-Leu-Phe-stimulated GTPγS binding and GTP hydrolysis, while plasma membrane density of formyl peptide receptors, α(i)2, α(i)3, β, γ5, and γ7 were not reduced. Reduced pertussis toxin-catalyzed ADP-ribosylation was re-established by in vitro methylation or addition of transducin βγ subunits. Superoxide release and inositol phosphate generation stimulated by fMet-Leu-Phe were significantly inhibited by Adox treatment. Carboxylmethylation contributes to transmembrane signalling and functional responses by enhancing association of α and βγ subunits.

Original languageEnglish (US)
Pages (from-to)1604-1614
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number3
DOIs
StatePublished - May 30 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Role of carboxylmethylation in chemoattractant receptor-stimulated G protein activation and functional responses'. Together they form a unique fingerprint.

Cite this