Role of CD34 family members in lumen formation in the developing kidney

Zhufeng Yang, Susan E. Zimmerman, Jun Tsunezumi, Caitlin Braitsch, Cary Trent, David M. Bryant, Ondine Cleaver, Consuelo González-Manchón, Denise K. Marciano

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron progenitors, and then relocalizes to the apical surface as progenitors epithelialize. Once an apical/luminal surface is formed, Endoglycan (and the actin-binding protein Ezrin) localize to large, intraluminal structures that may be vesicles/exosomes. We generated mice lacking Endoglycan and found mutants had timely initiation of lumen formation and continuous lumens, similar to controls. Mice with conditional deletion of both Endoglycan and Podocalyxin in developing nephrons also had normal tubular lumens. Despite this, Endoglycan/Podocalyxin is required for apical recruitment of the adaptor protein NHERF1, but not Ezrin, in podocyte precursors, a subset of the epithelia. In summary, while CD34 family members appear dispensable for lumen formation, our data identify Endoglycan as a novel pre-luminal marker and suggest lumen formation occurs via vesicular trafficking of apical cargo that includes Endoglycan.

Original languageEnglish (US)
Pages (from-to)66-74
Number of pages9
JournalDevelopmental Biology
Volume418
Issue number1
DOIs
StatePublished - Oct 1 2016

Keywords

  • CD34
  • Endoglycan
  • Kidney development
  • Lumen
  • Nephron
  • Podocalyxin
  • Polarity
  • Tubulogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Yang, Z., Zimmerman, S. E., Tsunezumi, J., Braitsch, C., Trent, C., Bryant, D. M., Cleaver, O., González-Manchón, C., & Marciano, D. K. (2016). Role of CD34 family members in lumen formation in the developing kidney. Developmental Biology, 418(1), 66-74. https://doi.org/10.1016/j.ydbio.2016.08.009