Role of DNA-PK in the cellular response to DNA double-strand breaks

Sandeep Burma, David J. Chen

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

The DNA-dependent protein kinase (DNA-PK) plays a critical role in DNA double-strand break (DSB) repair and in V(D)J recombination. DNA-PK also plays a very important role in triggering apoptosis in response to severe DNA damage or critically shortened telomeres. Paradoxically, components of the DNA-PK complex are present at the mammalian telomere where they function in capping chromosome ends to prevent them from being mistaken for double-strand breaks. In addition, DNA-PK appears to be involved in mounting an innate immune response to bacterial DNA and to viral infection. As DNA-PK localizes very rapidly to DNA breaks and phosphorylates itself and other damage-responsive proteins, it appears that DNA-PK serves as both a sensor and a transducer of DNA-damage signals. The many roles of DNA-PK in the mammalian cell are discussed in this review with particular emphasis on recent advances in our understanding of the phosphorylation events that take place during the activation of DNA-PK at DNA breaks.

Original languageEnglish (US)
Pages (from-to)909-918
Number of pages10
JournalDNA Repair
Volume3
Issue number8-9
DOIs
StatePublished - Aug 2004

Fingerprint

DNA-Activated Protein Kinase
Double-Stranded DNA Breaks
DNA
DNA Breaks
DNA Damage
V(D)J Recombination
Telomere Shortening
Bacterial DNA
Phosphorylation
Telomere
Virus Diseases
Chromosomes
Transducers
Mountings
Innate Immunity
Repair
Chemical activation
Cells
Apoptosis

Keywords

  • Apoptosis
  • DNA double-strand breaks
  • DNA-dependent protein kinase
  • Innate immunity
  • Non-homologous end joining
  • Phosphorylation
  • Telomere maintenance

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Role of DNA-PK in the cellular response to DNA double-strand breaks. / Burma, Sandeep; Chen, David J.

In: DNA Repair, Vol. 3, No. 8-9, 08.2004, p. 909-918.

Research output: Contribution to journalArticle

Burma, Sandeep ; Chen, David J. / Role of DNA-PK in the cellular response to DNA double-strand breaks. In: DNA Repair. 2004 ; Vol. 3, No. 8-9. pp. 909-918.
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