Role of Fas ligand in uveal melanoma-induced liver damage

A. C. Repp, E. S. Mayhew, K. Howard, H. Alizadeh, J. Y. Niederkorn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Uveal melanoma, the most common adult intraocular malignancy, metastasizes preferentially to the liver. Areas of cell death surrounding uveal melanoma metastases were observed in the livers of mice. We hypothesized that uveal melanoma cells might express Fas ligand (FasL), facilitating FasL-mediated apoptosis of Fas-expressing hepatocytes. Purpose: To determine whether Fas ligand (FasL)-expressing human uveal melanoma cells induce apoptosis of human hepatocytes in vitro and in vivo. Methods: Human uveal melanoma cell lines were assayed for FasL expression by flow cytometry and immunohistology. A human hepatocyte cell line was assayed for Fas expression by flow cytometry. Apoptosis of hepatocytes was detected by annexin V staining in vitro, and terminal deoxynucleotidyl transferase nick end labeling (TUNEL) in vivo. Results: Human uveal melanoma cell lines expressed FasL, as determined by flow cytometry and immunohistology. Human hepatocytes were Fas-positive by flow cytometry. In vitro, annexin V staining revealed that human uveal melanoma cells induced apoptosis of human hepatocytes. TUNEL staining of liver metastases revealed apoptosis of murine hepatocytes in contact with metastatic human uveal melanoma cells. Conclusion: FasL-induced apoptosis of hepatocytes in contact with FasL-positive human uveal melanoma cells may contribute to hepatic failure during metastatic disease.

Original languageEnglish (US)
Pages (from-to)752-758
Number of pages7
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume239
Issue number10
StatePublished - 2001

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Fas Ligand Protein
Hepatocytes
Liver
Apoptosis
Flow Cytometry
DNA Nucleotidylexotransferase
Annexin A5
Staining and Labeling
Cell Line
Uveal melanoma
Neoplasm Metastasis
Liver Failure
Cell Death

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Role of Fas ligand in uveal melanoma-induced liver damage. / Repp, A. C.; Mayhew, E. S.; Howard, K.; Alizadeh, H.; Niederkorn, J. Y.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 239, No. 10, 2001, p. 752-758.

Research output: Contribution to journalArticle

Repp, A. C. ; Mayhew, E. S. ; Howard, K. ; Alizadeh, H. ; Niederkorn, J. Y. / Role of Fas ligand in uveal melanoma-induced liver damage. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2001 ; Vol. 239, No. 10. pp. 752-758.
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