Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.

D. Schlossman, D. Withers, P. Welsh, A. Alexander, J. Robertus, A. Frankel

Research output: Contribution to journalArticle

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Abstract

The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.

Original languageEnglish (US)
Pages (from-to)5012-5021
Number of pages10
JournalMolecular and Cellular Biology
Volume9
Issue number11
StatePublished - Nov 1989

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Ricin
Ribosomes
Glutamic Acid
Mutant Proteins
beta-Galactosidase
Site-Directed Mutagenesis
Catalysis
Aspartic Acid
Alanine
Genes
Collagen
Escherichia coli
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Schlossman, D., Withers, D., Welsh, P., Alexander, A., Robertus, J., & Frankel, A. (1989). Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes. Molecular and Cellular Biology, 9(11), 5012-5021.

Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes. / Schlossman, D.; Withers, D.; Welsh, P.; Alexander, A.; Robertus, J.; Frankel, A.

In: Molecular and Cellular Biology, Vol. 9, No. 11, 11.1989, p. 5012-5021.

Research output: Contribution to journalArticle

Schlossman, D, Withers, D, Welsh, P, Alexander, A, Robertus, J & Frankel, A 1989, 'Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.', Molecular and Cellular Biology, vol. 9, no. 11, pp. 5012-5021.
Schlossman, D. ; Withers, D. ; Welsh, P. ; Alexander, A. ; Robertus, J. ; Frankel, A. / Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes. In: Molecular and Cellular Biology. 1989 ; Vol. 9, No. 11. pp. 5012-5021.
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