Role of glycosylation in the H-2-restricted cytolysis of virus-infected cells

P. L. Black, E. S. Vitetta, J. Forman, C. Y. Kang, R. D. May, J. W. Uhr

Research output: Contribution to journalArticle

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Abstract

The role of the oligosaccharide portions of cell surface glycoproteins in the susceptibility of virus-infected cells to H-2-restricted cytolysis was investigated by using the antibiotic tunicamycin (TM). TM inhibits the addition of sugars to the polypeptides of glycoproteins. TM treatment of P 815 cells before and during infection with vesicular stomatitis virus (VSV) inhibited glycosylation of proteins and reduced by about 50% the lysis of infected P 815 cells by VSV-immune, H-2-identical killer cells. In contrast, TM treatment had a modest inhibitory effect on cytolysis of P 815 cells by alloimmune effector cells. TM treatment did not inhibit the surface expression of either H-2 or VSV glycoprotein. Thus, glycosylation of H-2 and/or viral glycoprotein is a prerequisite for the lysis of infected cells by H-2-identical, VSV-immune cytotoxic cells.

Original languageEnglish (US)
Pages (from-to)48-55
Number of pages8
JournalEuropean Journal of Immunology
Volume11
Issue number1
DOIs
StatePublished - 1981

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Glycosylation
Tunicamycin
Viruses
Vesicular Stomatitis
Glycoproteins
Membrane Glycoproteins
Oligosaccharides
Anti-Bacterial Agents
Peptides
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Role of glycosylation in the H-2-restricted cytolysis of virus-infected cells. / Black, P. L.; Vitetta, E. S.; Forman, J.; Kang, C. Y.; May, R. D.; Uhr, J. W.

In: European Journal of Immunology, Vol. 11, No. 1, 1981, p. 48-55.

Research output: Contribution to journalArticle

Black, P. L. ; Vitetta, E. S. ; Forman, J. ; Kang, C. Y. ; May, R. D. ; Uhr, J. W. / Role of glycosylation in the H-2-restricted cytolysis of virus-infected cells. In: European Journal of Immunology. 1981 ; Vol. 11, No. 1. pp. 48-55.
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AU - May, R. D.

AU - Uhr, J. W.

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