Role of interferon-γ in a mouse model of allergic conjunctivitis

Michael E. Stern, Karyn Siemasko, Jianping Gao, Anh Duong, Clay Beauregard, Virginia Calder, Jerry Y. Niederkorn

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

PURPOSE. To characterize the effect of repeated topical exposure to allergen in a mouse model of allergic conjunctivitis and to determine the role of interferon-γ (TFN-γ) in the pathogenesis of allergic conjunctivitis. METHODS. Wild-type BALB/c mice and IFN-γ knockout (KO) BALB/c mice were sensitized in the footpad with short rag-weed (SRW) allergen and challenged topically for seven consecutive days with SRW allergen. The number of splenic CD4+ Th2 cells was determined by flow cytometry, and the cytokine profile of CD4+ T cells from SRW-sensitized mice was evaluated by ELISA. The role of IFN-γ in allergic conjunctivitis was also examined by timed in vivo neutralization with anti-IFN-γ antibody. Allergic conjunctivitis was evaluated clinically and histopathologically. RESULTS. Repeated topical challenge with SRW allergen induced allergic conjunctivitis that was characterized by lid edema, chemosis, redness, and tearing. Histopathological analysis revealed a marked conjunctival infiltrate that was predominantly neutrophils and eosinophils. IFN-γ KO mice and normal mice treated with anti-IFN-γ antibody displayed milder clinical symptoms of allergic conjunctivitis and a 70% reduction in the number of eosinophils that infiltrated the conjunctiva. Spleen cells from SRW-sensitized mice contained a large population of cells that expressed the Th2 surface marker T1/ST2 and produced IL-4, -5, and -10 and IFN-γ after stimulation with SRW allergen. CONCLUSIONS. Repeated topical application of SRW allergen induces a form of murine allergic conjunctivitis that mimics the human counterpart. IFN-γ appears to contribute to the pathogenesis of murine allergic conjunctivitis at the effector phase, but not during the initial sensitization stage.

Original languageEnglish (US)
Pages (from-to)3239-3246
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume46
Issue number9
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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