Role of macrophages in Acanthamoeba keratitis

F. Van Klink, W. M. Taylor, H. Alizadeh, M. J. Jager, N. Van Rooijen, J. Y. Niederkorn

Research output: Contribution to journalArticle

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Abstract

Purpose. Macrophages are believed to be the first line of defence in many infectious diseases, and are present in high numbers in corneas with Acanthamoeba keratitis, conjunctival macrophage depletion was performed in an animal model of Acanthamoeba infection to determine the importance of macrophages in this disease. Methods. Selective elimination of macrophages was achieved by repeated subconjunctival injection of liposomes containing dichloromethylene diphosphate in a Chinese hamster model of Acanthamoeba keratitis. Results. Macrophage depletion affected the incidence, severity, and chronicity of keratitis. The incidence of infection in normal animals was approximately 60% but rose to 100% in C12MDP-LIP treated animals at day four (N=24). Moreover, the clinical appearance of the keratitis in the C12MDP-LIP group was much more severe than the PBS-LIP group at all time points. There was also a major change in the chronicity of the keratitis, with an earlier onset and a prolonged and chronic course in the C12MDP-LIP treated hamsters. Conclusions. The profound exacerbation of Acanthamoeba keratitis in hamsters treated with C12MDP-LIP strongly suggests that macrophages play an important role in corneal infection with Acanthamoeba trophozoites probably by acting as a first line of defense and eliminate significant numbers of Acanthamoeba trophozoites.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Acanthamoeba Keratitis
Macrophages
Acanthamoeba
Keratitis
Trophozoites
Cricetinae
Infection
Diphosphates
Incidence
Cricetulus
Liposomes
Cornea
Communicable Diseases
Animal Models
Injections

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Van Klink, F., Taylor, W. M., Alizadeh, H., Jager, M. J., Van Rooijen, N., & Niederkorn, J. Y. (1996). Role of macrophages in Acanthamoeba keratitis. Investigative Ophthalmology and Visual Science, 37(3).

Role of macrophages in Acanthamoeba keratitis. / Van Klink, F.; Taylor, W. M.; Alizadeh, H.; Jager, M. J.; Van Rooijen, N.; Niederkorn, J. Y.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Van Klink, F, Taylor, WM, Alizadeh, H, Jager, MJ, Van Rooijen, N & Niederkorn, JY 1996, 'Role of macrophages in Acanthamoeba keratitis', Investigative Ophthalmology and Visual Science, vol. 37, no. 3.
Van Klink F, Taylor WM, Alizadeh H, Jager MJ, Van Rooijen N, Niederkorn JY. Role of macrophages in Acanthamoeba keratitis. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Van Klink, F. ; Taylor, W. M. ; Alizadeh, H. ; Jager, M. J. ; Van Rooijen, N. ; Niederkorn, J. Y. / Role of macrophages in Acanthamoeba keratitis. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
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