TY - JOUR
T1 - Role of Moringa oleifera in regulation of diabetes-induced oxidative stress
AU - Jaiswal, Dolly
AU - Rai, Prashant Kumar
AU - Mehta, Shikha
AU - Chatterji, Sanjukta
AU - Shukla, Surekha
AU - Rai, Devendra Kumar
AU - Sharma, Gaurav
AU - Sharma, Bechan
AU - khair, Shahidul
AU - Watal, Geeta
PY - 2013/6
Y1 - 2013/6
N2 - Objective: To evaluate the antioxidant activity of aqueous extract of. Moringa oleifera ( M. oleifera) young leaves by in vivo as well as in vitro assays. Methods: In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was done with the identified most effective dose of 200 mg/kg of its lyophilized powder on normal and diabetic rats. Its effect on different oxidative free radical scavenging enzymes,. viz, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), lipid peroxide (LPO) contents were measured. Results: Significant increase in activities of SOD, CAT, GST while, a decrease in LPO content was observed. Whereas, total phenolic, flavonoid and flavonol contents in the extract were found to be 120 mg/g of GAE, 40.5 mg/g of QE and 12.12 mg/g of QE, respectively. On the other hand, FRAP assay results of. M. oleifera leaves was (85.00±5.00)μM/g of extract powder. Conclusions: The significant antioxidant activities of. M. oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.
AB - Objective: To evaluate the antioxidant activity of aqueous extract of. Moringa oleifera ( M. oleifera) young leaves by in vivo as well as in vitro assays. Methods: In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was done with the identified most effective dose of 200 mg/kg of its lyophilized powder on normal and diabetic rats. Its effect on different oxidative free radical scavenging enzymes,. viz, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), lipid peroxide (LPO) contents were measured. Results: Significant increase in activities of SOD, CAT, GST while, a decrease in LPO content was observed. Whereas, total phenolic, flavonoid and flavonol contents in the extract were found to be 120 mg/g of GAE, 40.5 mg/g of QE and 12.12 mg/g of QE, respectively. On the other hand, FRAP assay results of. M. oleifera leaves was (85.00±5.00)μM/g of extract powder. Conclusions: The significant antioxidant activities of. M. oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.
KW - Antioxidant
KW - Diabetes
KW - Free radical
KW - Moringa oleifera leaves
KW - Oxidative damage
KW - Scavenging
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U2 - 10.1016/S1995-7645(13)60068-1
DO - 10.1016/S1995-7645(13)60068-1
M3 - Article
C2 - 23711700
AN - SCOPUS:84892414449
SN - 1995-7645
VL - 6
SP - 426
EP - 432
JO - Asian Pacific Journal of Tropical Medicine
JF - Asian Pacific Journal of Tropical Medicine
IS - 6
ER -