Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses

Randy J. Thresher, Martha Hotz Vitaterna, Yasuhide Miyamoto, Aleksey Kazantsev, David S. Hsu, Claude Petit, Christopher P. Selby, Lale Dawut, Oliver Smithies, Joseph S. Takahashi, Aziz Sancar

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.

Original languageEnglish (US)
Pages (from-to)1490-1494
Number of pages5
JournalScience
Volume282
Issue number5393
StatePublished - Nov 20 1998

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Cryptochromes
Suprachiasmatic Nucleus
Light
Circadian Clocks
Mammals
Messenger RNA
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Thresher, R. J., Vitaterna, M. H., Miyamoto, Y., Kazantsev, A., Hsu, D. S., Petit, C., ... Sancar, A. (1998). Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. Science, 282(5393), 1490-1494.

Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. / Thresher, Randy J.; Vitaterna, Martha Hotz; Miyamoto, Yasuhide; Kazantsev, Aleksey; Hsu, David S.; Petit, Claude; Selby, Christopher P.; Dawut, Lale; Smithies, Oliver; Takahashi, Joseph S.; Sancar, Aziz.

In: Science, Vol. 282, No. 5393, 20.11.1998, p. 1490-1494.

Research output: Contribution to journalArticle

Thresher, RJ, Vitaterna, MH, Miyamoto, Y, Kazantsev, A, Hsu, DS, Petit, C, Selby, CP, Dawut, L, Smithies, O, Takahashi, JS & Sancar, A 1998, 'Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses', Science, vol. 282, no. 5393, pp. 1490-1494.
Thresher RJ, Vitaterna MH, Miyamoto Y, Kazantsev A, Hsu DS, Petit C et al. Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. Science. 1998 Nov 20;282(5393):1490-1494.
Thresher, Randy J. ; Vitaterna, Martha Hotz ; Miyamoto, Yasuhide ; Kazantsev, Aleksey ; Hsu, David S. ; Petit, Claude ; Selby, Christopher P. ; Dawut, Lale ; Smithies, Oliver ; Takahashi, Joseph S. ; Sancar, Aziz. / Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. In: Science. 1998 ; Vol. 282, No. 5393. pp. 1490-1494.
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