Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses

Randy J. Thresher; Martha Hotz Vitaterna; Yasuhide Miyamoto; Aleksey Kazantsev; David S. Hsu; Claude Petit; Christopher P. Selby; Lale Dawut; Oliver Smithies; Joseph S. Takahashi; Aziz Sancar

doi:10.1126/science.282.5393.1490

Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses

Randy J. Thresher, Martha Hotz Vitaterna, Yasuhide Miyamoto, Aleksey Kazantsev, David S. Hsu, Claude Petit, Christopher P. Selby, Lale Dawut, Oliver Smithies, Joseph S. Takahashi, Aziz Sancar

Research output: Contribution to journal › Article › peer-review

332 Scopus citations

Abstract

Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.

Original language	English (US)
Pages (from-to)	1490-1494
Number of pages	5
Journal	Science
Volume	282
Issue number	5393
DOIs	https://doi.org/10.1126/science.282.5393.1490
State	Published - Nov 20 1998

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title = "Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses",

abstract = "Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.",

author = "Thresher, {Randy J.} and Vitaterna, {Martha Hotz} and Yasuhide Miyamoto and Aleksey Kazantsev and Hsu, {David S.} and Claude Petit and Selby, {Christopher P.} and Lale Dawut and Oliver Smithies and Takahashi, {Joseph S.} and Aziz Sancar",

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doi = "10.1126/science.282.5393.1490",

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AU - Thresher, Randy J.

AU - Vitaterna, Martha Hotz

AU - Miyamoto, Yasuhide

AU - Kazantsev, Aleksey

AU - Hsu, David S.

AU - Petit, Claude

AU - Selby, Christopher P.

AU - Dawut, Lale

AU - Smithies, Oliver

AU - Takahashi, Joseph S.

AU - Sancar, Aziz

PY - 1998/11/20

Y1 - 1998/11/20

N2 - Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.

AB - Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.

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Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses

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