Abstract
Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1490-1494 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 282 |
| Issue number | 5393 |
| State | Published - Nov 20 1998 |
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Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses. / Thresher, Randy J.; Vitaterna, Martha Hotz; Miyamoto, Yasuhide; Kazantsev, Aleksey; Hsu, David S.; Petit, Claude; Selby, Christopher P.; Dawut, Lale; Smithies, Oliver; Takahashi, Joseph S.; Sancar, Aziz.
In: Science, Vol. 282, No. 5393, 20.11.1998, p. 1490-1494.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses
AU - Thresher, Randy J.
AU - Vitaterna, Martha Hotz
AU - Miyamoto, Yasuhide
AU - Kazantsev, Aleksey
AU - Hsu, David S.
AU - Petit, Claude
AU - Selby, Christopher P.
AU - Dawut, Lale
AU - Smithies, Oliver
AU - Takahashi, Joseph S.
AU - Sancar, Aziz
PY - 1998/11/20
Y1 - 1998/11/20
N2 - Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.
AB - Cryptochromes are photoactive pigments in the eye that have been proposed to function as circadian photopigments. Mice lacking the cryptochrome 2 blue-light photoreceptor gene (mCry2) were tested for circadian clock-related functions. The mutant mice had a lower sensitivity to acute light induction of mPer1 in the suprachiasmatic nucleus (SCN) but exhibited normal circadian oscillations of mPer1 and mCry1 messenger RNA in the SCN. Behaviorally, the mutants had an intrinsic circadian period about 1 hour longer than normal and exhibited high-amplitude phase shifts in response to light pulses administered at circadian time 17. These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032553599&partnerID=8YFLogxK
M3 - Article
C2 - 9822380
AN - SCOPUS:0032553599
VL - 282
SP - 1490
EP - 1494
JO - Science
JF - Science
SN - 0036-8075
IS - 5393
ER -