Role of mucosal IgA in the resistance to Acanthamoeba keratitis

Henry F. Leher, Hassan Alizadeh, Wesley M. Taylor, Andrew S. Shea, Robert S. Silvany, Franciscus Van Klink, Martine J. Jager, Jerry Y. Niederkorn

Research output: Contribution to journalArticle

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Abstract

PURPOSE. To determine whether oral immunization with Acanthamoeba castellanii antigens elicits mucosal antibodies of the IgA isotype and whether mucosal antibodies affect parasite adhesion to the corneal epithelium. METHODS. Chinese hamsters were immunized with 100 μg aqueous Acanthamoeba antigen mixed with cholera toxin (Ac-CT) and subsequently challenged with parasite-laden contact lenses that were applied to abraded corneal surfaces. Tears and stool samples were examined for the presence of Acanthamoeba-specific IgA antibodies by enzyme-linked immunosorbent assay (ELISA). The effect of mucosal antibody on trophozoite binding to corneal epithelium and viability of trophozoites was examined in vitro. RESULTS. Hamsters immunized orally with Ac-CT showed significantly lower infection rates than did control groups (21.4% versus 72.6%). ELISA analysis of mucosal specimens showed the presence of parasite-specific IgA in stool samples and tears from hamsters orally immunized with Ac-CT, but not in control animals. In vitro assays showed that anti-Acanthamoeba IgA did not affect parasite viability. However, mucosal anti-Acanthamoeba IgA profoundly inhibited (>75%) the binding of parasites to corneal epithelial cells in vitro. CONCLUSIONS. Oral immunization with Ac-CT induces the production of parasite-specific IgA in mucosal secretions and prevents corneal infection. Mucosal antibody does not affect the viability of Acanthamoeba trophozoites but seems to prevent infection by inhibiting parasite binding to the corneal epithelium.

Original languageEnglish (US)
Pages (from-to)2666-2673
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume39
Issue number13
StatePublished - Dec 1998

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Acanthamoeba Keratitis
Acanthamoeba
Immunoglobulin A
Parasites
Trophozoites
Corneal Epithelium
Antibodies
Tears
Cricetinae
Immunization
Enzyme-Linked Immunosorbent Assay
Acanthamoeba castellanii
Antigens
Parasitic Diseases
Cholera Toxin
Contact Lenses
Infection Control
Cricetulus
Epithelial Cells
Control Groups

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Leher, H. F., Alizadeh, H., Taylor, W. M., Shea, A. S., Silvany, R. S., Van Klink, F., ... Niederkorn, J. Y. (1998). Role of mucosal IgA in the resistance to Acanthamoeba keratitis. Investigative Ophthalmology and Visual Science, 39(13), 2666-2673.

Role of mucosal IgA in the resistance to Acanthamoeba keratitis. / Leher, Henry F.; Alizadeh, Hassan; Taylor, Wesley M.; Shea, Andrew S.; Silvany, Robert S.; Van Klink, Franciscus; Jager, Martine J.; Niederkorn, Jerry Y.

In: Investigative Ophthalmology and Visual Science, Vol. 39, No. 13, 12.1998, p. 2666-2673.

Research output: Contribution to journalArticle

Leher, HF, Alizadeh, H, Taylor, WM, Shea, AS, Silvany, RS, Van Klink, F, Jager, MJ & Niederkorn, JY 1998, 'Role of mucosal IgA in the resistance to Acanthamoeba keratitis', Investigative Ophthalmology and Visual Science, vol. 39, no. 13, pp. 2666-2673.
Leher HF, Alizadeh H, Taylor WM, Shea AS, Silvany RS, Van Klink F et al. Role of mucosal IgA in the resistance to Acanthamoeba keratitis. Investigative Ophthalmology and Visual Science. 1998 Dec;39(13):2666-2673.
Leher, Henry F. ; Alizadeh, Hassan ; Taylor, Wesley M. ; Shea, Andrew S. ; Silvany, Robert S. ; Van Klink, Franciscus ; Jager, Martine J. ; Niederkorn, Jerry Y. / Role of mucosal IgA in the resistance to Acanthamoeba keratitis. In: Investigative Ophthalmology and Visual Science. 1998 ; Vol. 39, No. 13. pp. 2666-2673.
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AB - PURPOSE. To determine whether oral immunization with Acanthamoeba castellanii antigens elicits mucosal antibodies of the IgA isotype and whether mucosal antibodies affect parasite adhesion to the corneal epithelium. METHODS. Chinese hamsters were immunized with 100 μg aqueous Acanthamoeba antigen mixed with cholera toxin (Ac-CT) and subsequently challenged with parasite-laden contact lenses that were applied to abraded corneal surfaces. Tears and stool samples were examined for the presence of Acanthamoeba-specific IgA antibodies by enzyme-linked immunosorbent assay (ELISA). The effect of mucosal antibody on trophozoite binding to corneal epithelium and viability of trophozoites was examined in vitro. RESULTS. Hamsters immunized orally with Ac-CT showed significantly lower infection rates than did control groups (21.4% versus 72.6%). ELISA analysis of mucosal specimens showed the presence of parasite-specific IgA in stool samples and tears from hamsters orally immunized with Ac-CT, but not in control animals. In vitro assays showed that anti-Acanthamoeba IgA did not affect parasite viability. However, mucosal anti-Acanthamoeba IgA profoundly inhibited (>75%) the binding of parasites to corneal epithelial cells in vitro. CONCLUSIONS. Oral immunization with Ac-CT induces the production of parasite-specific IgA in mucosal secretions and prevents corneal infection. Mucosal antibody does not affect the viability of Acanthamoeba trophozoites but seems to prevent infection by inhibiting parasite binding to the corneal epithelium.

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