Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells

Farnaz Malekmarzban, Mohammad Seyedabadi, Mohammad Hossein Ghahremani, Shima Aliebrahimi, Peiman Habibollahi, Shohreh Tavajohi, Seyed Nasser Ostad

Research output: Contribution to journalArticle

Abstract

Cadmium (Cd) induces carcinogenicity and cytotoxicity through a variety of mechanisms. Metallothioneins (MTs) play critical roles in metal detoxification and radical scavenging. Here we evaluated the possible involvement of NO and MT in Cd-induced toxicity and resistance development. By utilizing Cd-resistant Caco-2 cells as a model of chronic exposure to Cadmium, we observed that Cd decreased Caco-2 cell proliferation, whereas Cd-resistant cells showed a lower sensitivity to Cd cytotoxicity. L-NAME as an iNOS inhibitor and cPTIO as an NO scavenger induced a significant reduction in Cd-mediated toxicity of parent Caco-2 in spite of resistant cells. In resistant cells, iNOS mRNA expression was declined; however, MT protein synthesis was increased following acute and chronic Cd exposure. It seems that NO synthesis involves in Cd-induced cytotoxicity, while elevated MT expression is associated with Cd detoxification and resistance.

Original languageEnglish (US)
Pages (from-to)287-294
Number of pages8
JournalActa Medica Iranica
Volume56
Issue number5
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Metallothionein
Cadmium
Nitric Oxide
Caco-2 Cells
NG-Nitroarginine Methyl Ester
Metals
Cell Proliferation

Keywords

  • Colon carcinoma
  • Detoxification
  • Free radical
  • Heavy metal
  • iNOS

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Malekmarzban, F., Seyedabadi, M., Ghahremani, M. H., Aliebrahimi, S., Habibollahi, P., Tavajohi, S., & Ostad, S. N. (2018). Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells. Acta Medica Iranica, 56(5), 287-294.

Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells. / Malekmarzban, Farnaz; Seyedabadi, Mohammad; Ghahremani, Mohammad Hossein; Aliebrahimi, Shima; Habibollahi, Peiman; Tavajohi, Shohreh; Ostad, Seyed Nasser.

In: Acta Medica Iranica, Vol. 56, No. 5, 01.01.2018, p. 287-294.

Research output: Contribution to journalArticle

Malekmarzban, F, Seyedabadi, M, Ghahremani, MH, Aliebrahimi, S, Habibollahi, P, Tavajohi, S & Ostad, SN 2018, 'Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells', Acta Medica Iranica, vol. 56, no. 5, pp. 287-294.
Malekmarzban F, Seyedabadi M, Ghahremani MH, Aliebrahimi S, Habibollahi P, Tavajohi S et al. Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells. Acta Medica Iranica. 2018 Jan 1;56(5):287-294.
Malekmarzban, Farnaz ; Seyedabadi, Mohammad ; Ghahremani, Mohammad Hossein ; Aliebrahimi, Shima ; Habibollahi, Peiman ; Tavajohi, Shohreh ; Ostad, Seyed Nasser. / Role of nitric oxide and metallothionein in cytotoxic activity of cadmium in CACO-2 cells. In: Acta Medica Iranica. 2018 ; Vol. 56, No. 5. pp. 287-294.
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