TY - JOUR
T1 - Role of non‐H‐2 antigens in the cytotoxic T cell response to allogeneic H‐2
AU - Langhorne, J.
AU - Lindahl, K. F.
PY - 1982
Y1 - 1982
N2 - A limiting dilution assay was used to compare the frequency of cytotoxic T lymphocyte precursors (CTL‐P) which respond to H‐2 antigens presented with additional background differences, with the frequency of CTL‐P which respond to H‐2 antigens on a self background. Individual cultures were divided and assayed for cytotoxic activity on the two targets sharing H‐2, but not the background; no cultures were seen which clearly killed one and not the other, and the same frequency of CTL‐P was measured on target cells that differed from the responder only at H‐2 and on target cells that differed also in the background, irrespective of the background of the stimulator. Thus, the assumption that allospecific cytotoxic T lymphocytes (CTL) recognize H‐2 plus minor histocompatibility antigens does not serve as an adequate explanation for the high frequency of allospecific CTL. The data also suggest that the two allelic forms of β2‐microglobulin do not contribute to the alloantigenic determinant.
AB - A limiting dilution assay was used to compare the frequency of cytotoxic T lymphocyte precursors (CTL‐P) which respond to H‐2 antigens presented with additional background differences, with the frequency of CTL‐P which respond to H‐2 antigens on a self background. Individual cultures were divided and assayed for cytotoxic activity on the two targets sharing H‐2, but not the background; no cultures were seen which clearly killed one and not the other, and the same frequency of CTL‐P was measured on target cells that differed from the responder only at H‐2 and on target cells that differed also in the background, irrespective of the background of the stimulator. Thus, the assumption that allospecific cytotoxic T lymphocytes (CTL) recognize H‐2 plus minor histocompatibility antigens does not serve as an adequate explanation for the high frequency of allospecific CTL. The data also suggest that the two allelic forms of β2‐microglobulin do not contribute to the alloantigenic determinant.
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U2 - 10.1002/eji.1830120202
DO - 10.1002/eji.1830120202
M3 - Article
C2 - 6210563
AN - SCOPUS:0020042951
SN - 0014-2980
VL - 12
SP - 101
EP - 106
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 2
ER -