Role of p120 Ras-GAP in directed cell movement

Sarang V. Kulkarni, Gerald Gish, Peter Van Der Geer, Mark Henkemeyer, Tony Pawson

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We have used cell lines deficient in p120 Ras GTPase activating protein (Ras-GAP) to investigate the roles of Ras-GAP and the associated p190 Rho-GAP (p190) in cell polarity and cell migration. Cell wounding assays showed that Ras-GAP-deficient cells were incapable of establishing complete cell polarity and migration into the wound. Stimulation of mutant cells with growth factor rescued defects in cell spreading, Golgi apparatus fragmentation, and polarized vesicular transport and partially rescued migration in a Ras- dependent manner. However, for directional movement, the turnover of stress fibers and focal adhesions to produce an elongate morphology was dependent on the constitutive association between Ras-GAP and p190, independent of Ras regulation. Disruption of the phosphotyrosine-mediated Ras-GAP/p190 complex by microinjecting synthetic peptides derived from p190 sequences in wild-type cells caused a suppression of actin filament reorientation and migration. From these observations we suggest that although Ras-GAP is not directly required for motility per se, it is important for cell polarization by regulating actin stress fiber and focal adhesion reorientation when complexed with 190. This observation suggests a specific function for Ras-GAP separate from Ras regulation in cell motility.

Original languageEnglish (US)
Pages (from-to)457-470
Number of pages14
JournalJournal of Cell Biology
Volume149
Issue number2
DOIs
StatePublished - Apr 17 2000

Fingerprint

p120 GTPase Activating Protein
ras GTPase-Activating Proteins
Cell Movement
Stress Fibers
Cell Polarity
Focal Adhesions
Phosphotyrosine
Golgi Apparatus
Actin Cytoskeleton
Actins
Intercellular Signaling Peptides and Proteins
Cell Line
Peptides

Keywords

  • Actin
  • Cell polarity
  • Cytoskeleton
  • Migration
  • p190 Rho-GAP

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kulkarni, S. V., Gish, G., Van Der Geer, P., Henkemeyer, M., & Pawson, T. (2000). Role of p120 Ras-GAP in directed cell movement. Journal of Cell Biology, 149(2), 457-470. https://doi.org/10.1083/jcb.149.2.457

Role of p120 Ras-GAP in directed cell movement. / Kulkarni, Sarang V.; Gish, Gerald; Van Der Geer, Peter; Henkemeyer, Mark; Pawson, Tony.

In: Journal of Cell Biology, Vol. 149, No. 2, 17.04.2000, p. 457-470.

Research output: Contribution to journalArticle

Kulkarni, SV, Gish, G, Van Der Geer, P, Henkemeyer, M & Pawson, T 2000, 'Role of p120 Ras-GAP in directed cell movement', Journal of Cell Biology, vol. 149, no. 2, pp. 457-470. https://doi.org/10.1083/jcb.149.2.457
Kulkarni, Sarang V. ; Gish, Gerald ; Van Der Geer, Peter ; Henkemeyer, Mark ; Pawson, Tony. / Role of p120 Ras-GAP in directed cell movement. In: Journal of Cell Biology. 2000 ; Vol. 149, No. 2. pp. 457-470.
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