Role of PHO85 gene in the cell cycle control of budding yeast

Y. F. Hu, X. H. Mao, C. D. Lu

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Abstract

By homo-recombination with yeast intergrating plasmids, a serial of haploid mutants of budding yeast YPH499 had been constructed, which included pho85 delta strain YPH600, pho85 delta cln1 delta strain YPH610, cln1 delta cln2 delta strain YPH640 and galactose inducible strain YPH630 (pho85 delta cln1 delta cln2 delta (GAL1-10PHO85)). By analyzing the growth rate of different strains, we concluded that PHO85 gene have greater influence than CLN1 and CLN2 genes on cell growth control. After transferred from galactose media to glucose media, the tri-mutant cells collected at intervals were observed with microscope and analyzed by FACS. The results showed that the tri-mutant cells arrest in G1 phase when they were transferred to glucose media.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalShi yan sheng wu xue bao
Volume31
Issue number3
StatePublished - Sep 1998

ASJC Scopus subject areas

  • Medicine(all)

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    Hu, Y. F., Mao, X. H., & Lu, C. D. (1998). Role of PHO85 gene in the cell cycle control of budding yeast. Shi yan sheng wu xue bao, 31(3), 265-271.