TY - JOUR
T1 - Role of the hepatocyte nuclear factor-1β (HNF-1β) C-terminal domain in Pkhd1 (ARPKD) gene transcription and renal cystogenesis
AU - Hiesberger, Thomas
AU - Shao, Xinli
AU - Gourley, Eric
AU - Reimann, Andreas
AU - Pontoglio, Marco
AU - Igarashi, Peter
PY - 2005/3/18
Y1 - 2005/3/18
N2 - Hepatocyte nuclear factor-1β (HNF-1β) is a homeodomain-containing transcription factor that regulates tissue-specific gene expression in the kidney and other epithelial organs. Mutations of HNF-1β produce congenital cystic abnormalities of the kidney, and previous studies showed that HNF-1β regulates the expression of the autosomal recessive polycystic kidney disease (ARPKD) gene, Pkhd1. Here we show that the C-terminal region of HNF-1β contains an activation domain that is functional when fused to a heterologous DNA-binding domain. An HNF-1β deletion mutant lacking the C-terminal domain interacts with wild-type HNF-1β, binds DNA, and functions as a dominant-negative inhibitor of a chromosomally integrated Pkhd1 promoter. The activation of the Pkhd1 promoter by wild-type HNF-1β is stimulated by sodium butyrate or coactivators CREB (cAMP-response element)-binding protein (CBP) and P/CAF. The interaction with CBP and P/CAF requires the C-terminal domain. Expression of an HNF-1β C-terminal deletion mutant in transgenic mice produces renal cysts, increased cell proliferation, and dilatation of the ureter similar to mice with kidney-specific inactivation of HNF-1β. Pkhd1 expression is inhibited in cystic collecting ducts but not in non-cystic proximal tubules, despite transgene expression in this nephron segment. We conclude that the C-terminal domain of HNF-1β is required for the activation of the Pkhd1 promoter. Deletion mutants lacking the C-terminal domain function as dominant-negative mutants, possibly by preventing the recruitment of histone acetylases to the promoter. Cyst formation correlates with inhibition of Pkhd1 expression, which argues that mutations of HNF-1β produce kidney cysts by down-regulating the ARPKD gene, Pkhd1. Expression of HNF-1α in proximal tubules may protect against cystogenesis.
AB - Hepatocyte nuclear factor-1β (HNF-1β) is a homeodomain-containing transcription factor that regulates tissue-specific gene expression in the kidney and other epithelial organs. Mutations of HNF-1β produce congenital cystic abnormalities of the kidney, and previous studies showed that HNF-1β regulates the expression of the autosomal recessive polycystic kidney disease (ARPKD) gene, Pkhd1. Here we show that the C-terminal region of HNF-1β contains an activation domain that is functional when fused to a heterologous DNA-binding domain. An HNF-1β deletion mutant lacking the C-terminal domain interacts with wild-type HNF-1β, binds DNA, and functions as a dominant-negative inhibitor of a chromosomally integrated Pkhd1 promoter. The activation of the Pkhd1 promoter by wild-type HNF-1β is stimulated by sodium butyrate or coactivators CREB (cAMP-response element)-binding protein (CBP) and P/CAF. The interaction with CBP and P/CAF requires the C-terminal domain. Expression of an HNF-1β C-terminal deletion mutant in transgenic mice produces renal cysts, increased cell proliferation, and dilatation of the ureter similar to mice with kidney-specific inactivation of HNF-1β. Pkhd1 expression is inhibited in cystic collecting ducts but not in non-cystic proximal tubules, despite transgene expression in this nephron segment. We conclude that the C-terminal domain of HNF-1β is required for the activation of the Pkhd1 promoter. Deletion mutants lacking the C-terminal domain function as dominant-negative mutants, possibly by preventing the recruitment of histone acetylases to the promoter. Cyst formation correlates with inhibition of Pkhd1 expression, which argues that mutations of HNF-1β produce kidney cysts by down-regulating the ARPKD gene, Pkhd1. Expression of HNF-1α in proximal tubules may protect against cystogenesis.
UR - http://www.scopus.com/inward/record.url?scp=15444375084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15444375084&partnerID=8YFLogxK
U2 - 10.1074/jbc.M414121200
DO - 10.1074/jbc.M414121200
M3 - Article
C2 - 15647252
AN - SCOPUS:15444375084
SN - 0021-9258
VL - 280
SP - 10578
EP - 10586
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 11
ER -