Role of TRPC3 in the formation of receptor- and store-operated calcium channels in A431 carcinoma cells

E. V. Kaznacheyeva, L. N. Glushankova, V. V. Bugaj, O. A. Zimina, A. Yu Skopin, V. A. Alexeenko, I. B. Bezprozvanny, G. N. Mozhayeva

Research output: Contribution to journalArticle

Abstract

Activation of PLC-linked intracellular signaling cascades in the non-excitable cells evokes the release of calcium ions from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca2+ stores and Ca2+ entry in the cytosol via Ca2+-channels of plasma membrane. The properties and molecular identity of these channels are now under intense investigation. It is speculated that mammalian proteins belonging to the TRP-related family take part in either receptor- and store- dependent entry, though data linking the specific TRP proteins and any endogenous Ca 2+- channel are very scarce. Thus we aimed to study the role of TRPC3 in the formation of receptor- and store- operated calcium entry pathways in A431 cells. Both whole-cell current recordings and fluorescent measurements of intracellular Ca2+ concentration have shown that partial inhibition of TRPC3 expression with small interfering RNAs (siRNA) suppresses the store-dependent Ca2+ entry, but does not affect the receptor-mediated Ca2+ entry. The investigations on the single-channel level revealed that TRPC3 suppression leads to the disappearance of one of the types of store operated Ca2+- channels in the plasma membrane and appearance of a new type of a store-independent channel in it. It might indicate that TRPC3 protein is needed for the functioning of store-dependent channel in A431 cells.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalBiologicheskie Membrany
Volume24
Issue number1
StatePublished - Mar 22 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Kaznacheyeva, E. V., Glushankova, L. N., Bugaj, V. V., Zimina, O. A., Skopin, A. Y., Alexeenko, V. A., Bezprozvanny, I. B., & Mozhayeva, G. N. (2007). Role of TRPC3 in the formation of receptor- and store-operated calcium channels in A431 carcinoma cells. Biologicheskie Membrany, 24(1), 87-96.