Role of tumor necrosis factor in preovulatory follicles of swine

J. Prange-Kiel, C. Kreutzkamm, U. Wehrenberg, G. M. Rune

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The effects of tumor necrosis factor (TNF) on cultured porcine granulosa cells that were obtained from preovulatory follicles were studied with regard to following parameters: 1) TNF receptor type I expression, 2) progesterone receptor and transforming growth factor β receptor type II (TβR II) as markers of luteinization, 3) proliferation, and 4) apoptosis. For comparative purposes the effects of TNF were also studied on insulin/forskolin-treated cells, as this treatment is well established to induce luteinization. Cytochemical methods followed by semiquantitative analysis were used. Our data show that TNF treatment upregulates TNF receptor type I expression in granulosa cells. TNF downregulates the expression of TβR II of insulin/forskolin-stimulated and of unstimulated cells. The progesterone receptor is also downregulated by the cytokine after insulin/forskolin-induced luteinization. Supplementation of the medium with TNF leads to increased proliferation and at the same time it induces apoptosis. Our results indicate that TNF exerts an inhibitory influence on luteinization and that TNF influences the balance between follicular growth (proliferation) and atresia (apoptosis).

Original languageEnglish (US)
Pages (from-to)928-935
Number of pages8
JournalBiology of Reproduction
Volume65
Issue number3
StatePublished - 2001

Fingerprint

Luteinization
Swine
Tumor Necrosis Factor-alpha
Colforsin
Receptors, Tumor Necrosis Factor, Type I
Granulosa Cells
Progesterone Receptors
Insulin
Apoptosis
Down-Regulation
Growth Factor Receptors
Transforming Growth Factors
Up-Regulation
Cytokines
Growth

Keywords

  • Apoptosis
  • Corpus luteum function
  • Follicular development
  • Granulosa cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Prange-Kiel, J., Kreutzkamm, C., Wehrenberg, U., & Rune, G. M. (2001). Role of tumor necrosis factor in preovulatory follicles of swine. Biology of Reproduction, 65(3), 928-935.

Role of tumor necrosis factor in preovulatory follicles of swine. / Prange-Kiel, J.; Kreutzkamm, C.; Wehrenberg, U.; Rune, G. M.

In: Biology of Reproduction, Vol. 65, No. 3, 2001, p. 928-935.

Research output: Contribution to journalArticle

Prange-Kiel, J, Kreutzkamm, C, Wehrenberg, U & Rune, GM 2001, 'Role of tumor necrosis factor in preovulatory follicles of swine', Biology of Reproduction, vol. 65, no. 3, pp. 928-935.
Prange-Kiel, J. ; Kreutzkamm, C. ; Wehrenberg, U. ; Rune, G. M. / Role of tumor necrosis factor in preovulatory follicles of swine. In: Biology of Reproduction. 2001 ; Vol. 65, No. 3. pp. 928-935.
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