Abstract
Cytokinesis is a complicated yet conserved step of the cell-division cycle that requires the coordination of multiple proteins and cellular processes. Here we describe a previously uncharacterized protein, Ync13, and its roles during fission yeast cytokinesis. Ync13 is a member of the UNC-13/Munc13 protein family, whose animal homologues are essential priming factors for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex assembly during exocytosis in various cell types, but no roles in cytokinesis have been reported. We find that Ync13 binds to lipids in vitro and dynamically localizes to the plasma membrane at cell tips during interphase and at the division site during cytokinesis. Deletion of Ync13 leads to defective septation and exocytosis, uneven distribution of cell-wall enzymes and components of cell-wall integrity pathway along the division site and massive cell lysis during cell separation. Interestingly, loss of Ync13 compromises endocytic site selection at the division plane. Collectively, we find that Ync13 has a novel function as an UNC-13/Munc13 protein in coordinating exocytosis, endocytosis, and cell-wall integrity during fission yeast cytokinesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2259-2279 |
| Number of pages | 21 |
| Journal | Molecular Biology of the Cell |
| Volume | 29 |
| Issue number | 19 |
| DOIs | |
| State | Published - Sep 15 2018 |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis. / Zhu, Yi Hua; Hyun, Joanne; Pan, Yun Zu; Hopper, James E.; Rizo-Rey, Jose; Wu, Jian Qiu.
In: Molecular Biology of the Cell, Vol. 29, No. 19, 15.09.2018, p. 2259-2279.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis
AU - Zhu, Yi Hua
AU - Hyun, Joanne
AU - Pan, Yun Zu
AU - Hopper, James E.
AU - Rizo-Rey, Jose
AU - Wu, Jian Qiu
PY - 2018/9/15
Y1 - 2018/9/15
N2 - Cytokinesis is a complicated yet conserved step of the cell-division cycle that requires the coordination of multiple proteins and cellular processes. Here we describe a previously uncharacterized protein, Ync13, and its roles during fission yeast cytokinesis. Ync13 is a member of the UNC-13/Munc13 protein family, whose animal homologues are essential priming factors for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex assembly during exocytosis in various cell types, but no roles in cytokinesis have been reported. We find that Ync13 binds to lipids in vitro and dynamically localizes to the plasma membrane at cell tips during interphase and at the division site during cytokinesis. Deletion of Ync13 leads to defective septation and exocytosis, uneven distribution of cell-wall enzymes and components of cell-wall integrity pathway along the division site and massive cell lysis during cell separation. Interestingly, loss of Ync13 compromises endocytic site selection at the division plane. Collectively, we find that Ync13 has a novel function as an UNC-13/Munc13 protein in coordinating exocytosis, endocytosis, and cell-wall integrity during fission yeast cytokinesis.
AB - Cytokinesis is a complicated yet conserved step of the cell-division cycle that requires the coordination of multiple proteins and cellular processes. Here we describe a previously uncharacterized protein, Ync13, and its roles during fission yeast cytokinesis. Ync13 is a member of the UNC-13/Munc13 protein family, whose animal homologues are essential priming factors for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex assembly during exocytosis in various cell types, but no roles in cytokinesis have been reported. We find that Ync13 binds to lipids in vitro and dynamically localizes to the plasma membrane at cell tips during interphase and at the division site during cytokinesis. Deletion of Ync13 leads to defective septation and exocytosis, uneven distribution of cell-wall enzymes and components of cell-wall integrity pathway along the division site and massive cell lysis during cell separation. Interestingly, loss of Ync13 compromises endocytic site selection at the division plane. Collectively, we find that Ync13 has a novel function as an UNC-13/Munc13 protein in coordinating exocytosis, endocytosis, and cell-wall integrity during fission yeast cytokinesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=85053423290&partnerID=8YFLogxK
U2 - 10.1091/mbc.E18-04-0225
DO - 10.1091/mbc.E18-04-0225
M3 - Article
C2 - 30044717
AN - SCOPUS:85053423290
VL - 29
SP - 2259
EP - 2279
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
SN - 1059-1524
IS - 19
ER -