Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis

Yi Hua Zhu, Joanne Hyun, Yun Zu Pan, James E. Hopper, Jose Rizo-Rey, Jian Qiu Wu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cytokinesis is a complicated yet conserved step of the cell-division cycle that requires the coordination of multiple proteins and cellular processes. Here we describe a previously uncharacterized protein, Ync13, and its roles during fission yeast cytokinesis. Ync13 is a member of the UNC-13/Munc13 protein family, whose animal homologues are essential priming factors for soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex assembly during exocytosis in various cell types, but no roles in cytokinesis have been reported. We find that Ync13 binds to lipids in vitro and dynamically localizes to the plasma membrane at cell tips during interphase and at the division site during cytokinesis. Deletion of Ync13 leads to defective septation and exocytosis, uneven distribution of cell-wall enzymes and components of cell-wall integrity pathway along the division site and massive cell lysis during cell separation. Interestingly, loss of Ync13 compromises endocytic site selection at the division plane. Collectively, we find that Ync13 has a novel function as an UNC-13/Munc13 protein in coordinating exocytosis, endocytosis, and cell-wall integrity during fission yeast cytokinesis.

Original languageEnglish (US)
Pages (from-to)2259-2279
Number of pages21
JournalMolecular Biology of the Cell
Volume29
Issue number19
DOIs
StatePublished - Sep 15 2018

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Cytokinesis
Schizosaccharomyces
Exocytosis
Cell Wall
Proteins
SNARE Proteins
Cell Separation
Interphase
Cellular Structures
Endocytosis
Cell Cycle
Cell Membrane
Lipids
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis. / Zhu, Yi Hua; Hyun, Joanne; Pan, Yun Zu; Hopper, James E.; Rizo-Rey, Jose; Wu, Jian Qiu.

In: Molecular Biology of the Cell, Vol. 29, No. 19, 15.09.2018, p. 2259-2279.

Research output: Contribution to journalArticle

Zhu, Yi Hua ; Hyun, Joanne ; Pan, Yun Zu ; Hopper, James E. ; Rizo-Rey, Jose ; Wu, Jian Qiu. / Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis. In: Molecular Biology of the Cell. 2018 ; Vol. 29, No. 19. pp. 2259-2279.
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