TY - JOUR
T1 - Root dentin body moisture changes caused by ascorbic acid
AU - Komabayashi, Takashi
AU - Ahn, Chul
AU - Zhang, Song
AU - Zhu, Qiang
AU - Spångberg, Larz S W
N1 - Funding Information:
The authors thank Ms. Jeanne Santa Cruz (Texas A&M Health Science Center Baylor College of Dentistry) for the critical editing. This publication was supported by Grant Number NIH KL2RR024983 (TK) and UL1 RR024982, entitled, “North and Central Texas Clinical and Translational Science Initiative” (Milton Packer, M.D., PI) from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research, and its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NCRR or NIH. Information on NCRR is available at http://www.ncrr.nih.gov/ . Information on Re-engineering the Clinical Research Enterprise can be obtained from http://nihroadmap.nih.gov/clinicalresearch/overview-translational.asp .”
PY - 2009/6
Y1 - 2009/6
N2 - Objectives: To quantify in vitro the root dentin moisture (DM) when soaked in 10% ascorbic acid solution (Group A) and distilled water (Group B) for up to 14 days. Methods: Forty-four extracted human mandibular single-rooted teeth were sectioned perpendicular to the long axis at the CEJ to access the root dentin. The samples were divided into Groups A and B. Baseline (day 0) DM was measured using a digital grain moisture meter. One sample was placed in each tube, which was then filled with solution. All tubes were kept at 37 °C and 100% humidity. DM was measured after 1, 3, 7, and 14 days. The baseline DM value was used as a covariate in the generalized estimating equation (GEE) analysis to account for the difference in the baseline DM between the two groups. Results: The mean DM (%) values ± standard deviation on days 0, 1, 3, 7, and 14 were 11.4 ± 1.08, 17.1 ± 0.87, 18.2 ± 0.76, 18.4 ± 0.77, and 17.9 ± 0.90 in Group A, and 10.2 ± 0.95, 12.8 ± 0.90, 13.3 ± 0.95, 13.0 ± 0.91, and 13.2 ± 0.89 in Group B, respectively. Group A had significantly higher baseline DM than Group B (p = 0.006). After adjusting for differences in the baseline, the GEE analysis indicated that, on average, Group A had a significantly higher increase in DM than Group B, with means ± standard deviation of 4.35 ± 0.26. Conclusions: The moisture increase in the ascorbic acid group was greater than that in the distilled water group. Soaking root dentin in the unreplenished ascorbic acid solution or distilled water beyond 3 days did not further increase DM.
AB - Objectives: To quantify in vitro the root dentin moisture (DM) when soaked in 10% ascorbic acid solution (Group A) and distilled water (Group B) for up to 14 days. Methods: Forty-four extracted human mandibular single-rooted teeth were sectioned perpendicular to the long axis at the CEJ to access the root dentin. The samples were divided into Groups A and B. Baseline (day 0) DM was measured using a digital grain moisture meter. One sample was placed in each tube, which was then filled with solution. All tubes were kept at 37 °C and 100% humidity. DM was measured after 1, 3, 7, and 14 days. The baseline DM value was used as a covariate in the generalized estimating equation (GEE) analysis to account for the difference in the baseline DM between the two groups. Results: The mean DM (%) values ± standard deviation on days 0, 1, 3, 7, and 14 were 11.4 ± 1.08, 17.1 ± 0.87, 18.2 ± 0.76, 18.4 ± 0.77, and 17.9 ± 0.90 in Group A, and 10.2 ± 0.95, 12.8 ± 0.90, 13.3 ± 0.95, 13.0 ± 0.91, and 13.2 ± 0.89 in Group B, respectively. Group A had significantly higher baseline DM than Group B (p = 0.006). After adjusting for differences in the baseline, the GEE analysis indicated that, on average, Group A had a significantly higher increase in DM than Group B, with means ± standard deviation of 4.35 ± 0.26. Conclusions: The moisture increase in the ascorbic acid group was greater than that in the distilled water group. Soaking root dentin in the unreplenished ascorbic acid solution or distilled water beyond 3 days did not further increase DM.
KW - Ascorbic acid
KW - Bonding
KW - Dentin moisture
KW - Generalized estimating equation analysis
KW - Rapid non-destructive method
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U2 - 10.1016/j.jdent.2009.03.004
DO - 10.1016/j.jdent.2009.03.004
M3 - Article
C2 - 19346052
AN - SCOPUS:64549158796
SN - 0300-5712
VL - 37
SP - 475
EP - 479
JO - Journal of Dentistry
JF - Journal of Dentistry
IS - 6
ER -