RPA-like proteins mediate yeast telomere function

Hua Gao, Rachel B. Cervantes, Edward K. Mandell, Joel H. Otero, Victoria Lundblad

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

Cdc13, Stn1 and Ten1 are essential yeast proteins that both protect chromosome termini from unregulated resection and regulate telomere length. Cdc13, which localizes to telomeres through high-affinity binding to telomeric single-stranded DNA, has been extensively characterized, whereas the contribution(s) of the Cdc13-associated Stn1 and Ten1 proteins to telomere function have remained unclear. We show here that Stn1 and Ten1 are DNA-binding proteins with specificity for telomeric DNA substrates. Furthermore, Stn1 and Ten1 show similarities to Rpa2 and Rpa3, subunits of the heterotrimeric replication protein A (RPA) complex, which is the major single-stranded DNA-binding activity in eukaryotic cells. We propose that Cdc13, Stn1 and Ten1 function as a telomere-specific RPA-like complex. Identification of an RPA-like complex that is targeted to a specific region of the genome suggests that multiple RPA-like complexes have evolved, each making individual contributions to genomic stability.

Original languageEnglish (US)
Pages (from-to)208-214
Number of pages7
JournalNature Structural and Molecular Biology
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2007

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Gao, H., Cervantes, R. B., Mandell, E. K., Otero, J. H., & Lundblad, V. (2007). RPA-like proteins mediate yeast telomere function. Nature Structural and Molecular Biology, 14(3), 208-214. https://doi.org/10.1038/nsmb1205