RraA: A protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli

Kangseok Lee, Xiaoming Zhan, Junjun Gao, Ji Qiu, Yanan Feng, R. Meganathan, Stanley N. Cohen, George Georgiou

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Ribonuclease E (RNase E) has a key role in mRNA degradation and the processing of catalytic and structural RNAs in E. coli. We report the discovery of an evolutionarily conserved 17.4 kDa protein, here named RraA (regulator of ribonuclease activity A) that binds to RNase E and inhibits RNase E endonucleolytic cleavages without altering cleavage site specificity or interacting detectably with substrate RNAs. Overexpression of RraA circumvents the effects of an autoregulatory mechanism that normally maintains the RNase E cellular level within a narrow range, resulting in the genome-wide accumulation of RNase E-targeted transcripts. While not required for RraA action, the C-terminal RNase E region that serves as a scaffold for formation of a multiprotein degradosome complex modulates the inhibition of RNase E catalytic activity by RraA. Our results reveal a possible mechanism for the dynamic regulation of RNA decay and processing by inhibitory RNase binding proteins.

Original languageEnglish (US)
Pages (from-to)623-634
Number of pages12
JournalCell
Volume114
Issue number5
DOIs
StatePublished - Sep 5 2003

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Ribonucleases
Escherichia coli
RNA
Proteins
RNA Stability
E region
Multiprotein Complexes
Catalytic RNA
Processing
ribonuclease E
Scaffolds
Catalyst activity
Carrier Proteins
Genes
Genome
Degradation
Messenger RNA
Substrates

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

RraA : A protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli. / Lee, Kangseok; Zhan, Xiaoming; Gao, Junjun; Qiu, Ji; Feng, Yanan; Meganathan, R.; Cohen, Stanley N.; Georgiou, George.

In: Cell, Vol. 114, No. 5, 05.09.2003, p. 623-634.

Research output: Contribution to journalArticle

Lee, K, Zhan, X, Gao, J, Qiu, J, Feng, Y, Meganathan, R, Cohen, SN & Georgiou, G 2003, 'RraA: A protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli', Cell, vol. 114, no. 5, pp. 623-634. https://doi.org/10.1016/j.cell.2003.08.003
Lee, Kangseok ; Zhan, Xiaoming ; Gao, Junjun ; Qiu, Ji ; Feng, Yanan ; Meganathan, R. ; Cohen, Stanley N. ; Georgiou, George. / RraA : A protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli. In: Cell. 2003 ; Vol. 114, No. 5. pp. 623-634.
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