S3-12, adipophilin, and TIP47 package lipid in adipocytes

Nathan E. Wolins, Benjamin K. Quaynor, James R. Skinner, Marissa J. Schoenfish, Anatoly Tzekov, Perry E. Bickel

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

Animals have evolved mechanisms to maintain circulating nutrient levels when energy demands exceed feeding opportunities. Mammals store most of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes. How newly synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood. Perilipin is a member of the evolutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequence similarity and association with lipid droplets. We previously showed that 83-12, which is also a member of this family, associates with a separate pool of lipid droplets that emerge when triacylglycerol storage is driven by adding oleate to the culture medium of adipocytes. Our current data extend these findings to demonstrate that nascent lipid droplets emerge with a coat composed of 33-12, TIP47, and adipophilin. After 100 min of oleate treatment, the nascent lipid droplets are more heterogeneous: 83-12 and TIP47 coat smaller, peripheral droplets and adipophilin coats a more medial population of droplets. Fractionation of untreated and oleate-treated adipocytes shows oleate-dependent redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction. Inhibition of protein synthesis with cycloheximide does not block the oleate-induced formation of the nascent lipid droplets, nor does it prevent TAG accumulation. We suggest that the non-lipid droplet pools of 83-12, adipophilin, and TIP47 constitute a ready reservoir of coat proteins to permit rapid packaging of newly synthesized triacylglycerol and to maximize energy storage during nutrient excess.

Original languageEnglish (US)
Pages (from-to)19146-19155
Number of pages10
JournalJournal of Biological Chemistry
Volume280
Issue number19
DOIs
StatePublished - May 13 2005

Fingerprint

Adipocytes
Lipids
Oleic Acid
Triglycerides
Nutrients
Food
Perilipin-2
Lipid Droplets
Capsid Proteins
Product Packaging
Cycloheximide
Culture Media
Mammals
Fractionation
Energy storage
Electron energy levels
Packaging
Animals
Proteins
Perilipin-1

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wolins, N. E., Quaynor, B. K., Skinner, J. R., Schoenfish, M. J., Tzekov, A., & Bickel, P. E. (2005). S3-12, adipophilin, and TIP47 package lipid in adipocytes. Journal of Biological Chemistry, 280(19), 19146-19155. https://doi.org/10.1074/jbc.M500978200

S3-12, adipophilin, and TIP47 package lipid in adipocytes. / Wolins, Nathan E.; Quaynor, Benjamin K.; Skinner, James R.; Schoenfish, Marissa J.; Tzekov, Anatoly; Bickel, Perry E.

In: Journal of Biological Chemistry, Vol. 280, No. 19, 13.05.2005, p. 19146-19155.

Research output: Contribution to journalArticle

Wolins, NE, Quaynor, BK, Skinner, JR, Schoenfish, MJ, Tzekov, A & Bickel, PE 2005, 'S3-12, adipophilin, and TIP47 package lipid in adipocytes', Journal of Biological Chemistry, vol. 280, no. 19, pp. 19146-19155. https://doi.org/10.1074/jbc.M500978200
Wolins NE, Quaynor BK, Skinner JR, Schoenfish MJ, Tzekov A, Bickel PE. S3-12, adipophilin, and TIP47 package lipid in adipocytes. Journal of Biological Chemistry. 2005 May 13;280(19):19146-19155. https://doi.org/10.1074/jbc.M500978200
Wolins, Nathan E. ; Quaynor, Benjamin K. ; Skinner, James R. ; Schoenfish, Marissa J. ; Tzekov, Anatoly ; Bickel, Perry E. / S3-12, adipophilin, and TIP47 package lipid in adipocytes. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 19. pp. 19146-19155.
@article{ba3ba681a3144930ae1aa2d7c212e029,
title = "S3-12, adipophilin, and TIP47 package lipid in adipocytes",
abstract = "Animals have evolved mechanisms to maintain circulating nutrient levels when energy demands exceed feeding opportunities. Mammals store most of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes. How newly synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood. Perilipin is a member of the evolutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequence similarity and association with lipid droplets. We previously showed that 83-12, which is also a member of this family, associates with a separate pool of lipid droplets that emerge when triacylglycerol storage is driven by adding oleate to the culture medium of adipocytes. Our current data extend these findings to demonstrate that nascent lipid droplets emerge with a coat composed of 33-12, TIP47, and adipophilin. After 100 min of oleate treatment, the nascent lipid droplets are more heterogeneous: 83-12 and TIP47 coat smaller, peripheral droplets and adipophilin coats a more medial population of droplets. Fractionation of untreated and oleate-treated adipocytes shows oleate-dependent redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction. Inhibition of protein synthesis with cycloheximide does not block the oleate-induced formation of the nascent lipid droplets, nor does it prevent TAG accumulation. We suggest that the non-lipid droplet pools of 83-12, adipophilin, and TIP47 constitute a ready reservoir of coat proteins to permit rapid packaging of newly synthesized triacylglycerol and to maximize energy storage during nutrient excess.",
author = "Wolins, {Nathan E.} and Quaynor, {Benjamin K.} and Skinner, {James R.} and Schoenfish, {Marissa J.} and Anatoly Tzekov and Bickel, {Perry E.}",
year = "2005",
month = "5",
day = "13",
doi = "10.1074/jbc.M500978200",
language = "English (US)",
volume = "280",
pages = "19146--19155",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "19",

}

TY - JOUR

T1 - S3-12, adipophilin, and TIP47 package lipid in adipocytes

AU - Wolins, Nathan E.

AU - Quaynor, Benjamin K.

AU - Skinner, James R.

AU - Schoenfish, Marissa J.

AU - Tzekov, Anatoly

AU - Bickel, Perry E.

PY - 2005/5/13

Y1 - 2005/5/13

N2 - Animals have evolved mechanisms to maintain circulating nutrient levels when energy demands exceed feeding opportunities. Mammals store most of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes. How newly synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood. Perilipin is a member of the evolutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequence similarity and association with lipid droplets. We previously showed that 83-12, which is also a member of this family, associates with a separate pool of lipid droplets that emerge when triacylglycerol storage is driven by adding oleate to the culture medium of adipocytes. Our current data extend these findings to demonstrate that nascent lipid droplets emerge with a coat composed of 33-12, TIP47, and adipophilin. After 100 min of oleate treatment, the nascent lipid droplets are more heterogeneous: 83-12 and TIP47 coat smaller, peripheral droplets and adipophilin coats a more medial population of droplets. Fractionation of untreated and oleate-treated adipocytes shows oleate-dependent redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction. Inhibition of protein synthesis with cycloheximide does not block the oleate-induced formation of the nascent lipid droplets, nor does it prevent TAG accumulation. We suggest that the non-lipid droplet pools of 83-12, adipophilin, and TIP47 constitute a ready reservoir of coat proteins to permit rapid packaging of newly synthesized triacylglycerol and to maximize energy storage during nutrient excess.

AB - Animals have evolved mechanisms to maintain circulating nutrient levels when energy demands exceed feeding opportunities. Mammals store most of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes. How newly synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood. Perilipin is a member of the evolutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequence similarity and association with lipid droplets. We previously showed that 83-12, which is also a member of this family, associates with a separate pool of lipid droplets that emerge when triacylglycerol storage is driven by adding oleate to the culture medium of adipocytes. Our current data extend these findings to demonstrate that nascent lipid droplets emerge with a coat composed of 33-12, TIP47, and adipophilin. After 100 min of oleate treatment, the nascent lipid droplets are more heterogeneous: 83-12 and TIP47 coat smaller, peripheral droplets and adipophilin coats a more medial population of droplets. Fractionation of untreated and oleate-treated adipocytes shows oleate-dependent redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction. Inhibition of protein synthesis with cycloheximide does not block the oleate-induced formation of the nascent lipid droplets, nor does it prevent TAG accumulation. We suggest that the non-lipid droplet pools of 83-12, adipophilin, and TIP47 constitute a ready reservoir of coat proteins to permit rapid packaging of newly synthesized triacylglycerol and to maximize energy storage during nutrient excess.

UR - http://www.scopus.com/inward/record.url?scp=21444437133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21444437133&partnerID=8YFLogxK

U2 - 10.1074/jbc.M500978200

DO - 10.1074/jbc.M500978200

M3 - Article

C2 - 15731108

AN - SCOPUS:21444437133

VL - 280

SP - 19146

EP - 19155

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 19

ER -