Schedule-dependent cytotoxicity of methotrexate and 5-fluorouracil in human colon and breast tumor cell lines

C. Benz, M. Schoenberg, M. Choti, E. Cadman

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The effect of sequential methotrexate and 5-fluorouracil on the clonal growth of the human colon adenocarcinoma cell HCT-8, and the hormone-dependent human breast carcinoma cell, 47-DN, was examined. In both cell lines, when 5-fluorouracil was given during the last 6 h of a 24-h methotrexate exposure period, there was marked synergistic inhibition of clonal growth. Shorter intervals or the reverse sequence of drugs were either additive or antagonistic. These results indicate the importance of the drug sequence and time interval between drug administration for optimal cytotoxicity in these human cell lines. This information suggests that the administration of methotrexate 18 h before 5-fluorouracil may have potential application in the design of clinical trials for these malignancies.

Original languageEnglish (US)
Pages (from-to)1162-1165
Number of pages4
JournalJournal of Clinical Investigation
Volume66
Issue number5
StatePublished - 1980

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Tumor Cell Line
Methotrexate
Fluorouracil
Appointments and Schedules
Colon
Breast Neoplasms
Pharmaceutical Preparations
Cell Line
Growth
Adenocarcinoma
Clinical Trials
Hormones
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Schedule-dependent cytotoxicity of methotrexate and 5-fluorouracil in human colon and breast tumor cell lines. / Benz, C.; Schoenberg, M.; Choti, M.; Cadman, E.

In: Journal of Clinical Investigation, Vol. 66, No. 5, 1980, p. 1162-1165.

Research output: Contribution to journalArticle

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AB - The effect of sequential methotrexate and 5-fluorouracil on the clonal growth of the human colon adenocarcinoma cell HCT-8, and the hormone-dependent human breast carcinoma cell, 47-DN, was examined. In both cell lines, when 5-fluorouracil was given during the last 6 h of a 24-h methotrexate exposure period, there was marked synergistic inhibition of clonal growth. Shorter intervals or the reverse sequence of drugs were either additive or antagonistic. These results indicate the importance of the drug sequence and time interval between drug administration for optimal cytotoxicity in these human cell lines. This information suggests that the administration of methotrexate 18 h before 5-fluorouracil may have potential application in the design of clinical trials for these malignancies.

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