Scm3 Is Essential to Recruit the Histone H3 Variant Cse4 to Centromeres and to Maintain a Functional Kinetochore

Raymond Camahort, Bing Li, Laurence Florens, Selene K. Swanson, Michael P. Washburn, Jennifer L. Gerton

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

The kinetochore is a complex multiprotein structure located at centromeres that is essential for proper chromosome segregation. Budding-yeast Cse4 is an essential evolutionarily conserved histone H3 variant recruited to the centromere by an unknown mechanism. We have identified Scm3, an inner kinetochore protein that immunopurifies with Cse4. Scm3 is essential for viability and localizes to all centromeres. Construction of a conditional SCM3 allele reveals that depletion results in metaphase arrest, with duplicated spindle poles, short spindles, and unequal DNA distribution. The metaphase arrest is mediated by the mitotic spindle checkpoint being dependent on Mad1 and the Aurora kinase B homolog Ipl1. Scm3 interacts with both Ndc10 and Cse4 and is essential to establish centromeric chromatin after DNA replication. In addition, Scm3 is required to maintain kinetochore function throughout the cell cycle. We propose a model in which Ndc10/Scm3 binds to centromeric DNA, which is in turn essential for targeting Cse4 to centromeres.

Original languageEnglish (US)
Pages (from-to)853-865
Number of pages13
JournalMolecular Cell
Volume26
Issue number6
DOIs
StatePublished - Jun 22 2007

Fingerprint

Kinetochores
Centromere
Histones
Metaphase
Aurora Kinase B
Spindle Poles
M Phase Cell Cycle Checkpoints
Multiprotein Complexes
Chromosome Segregation
Saccharomycetales
DNA
DNA Replication
Chromatin
Cell Cycle
Alleles
Proteins

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Scm3 Is Essential to Recruit the Histone H3 Variant Cse4 to Centromeres and to Maintain a Functional Kinetochore. / Camahort, Raymond; Li, Bing; Florens, Laurence; Swanson, Selene K.; Washburn, Michael P.; Gerton, Jennifer L.

In: Molecular Cell, Vol. 26, No. 6, 22.06.2007, p. 853-865.

Research output: Contribution to journalArticle

Camahort, Raymond ; Li, Bing ; Florens, Laurence ; Swanson, Selene K. ; Washburn, Michael P. ; Gerton, Jennifer L. / Scm3 Is Essential to Recruit the Histone H3 Variant Cse4 to Centromeres and to Maintain a Functional Kinetochore. In: Molecular Cell. 2007 ; Vol. 26, No. 6. pp. 853-865.
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