@article{86265192525c449d8eef87b6da22c4be,
title = "Scm3 Is Essential to Recruit the Histone H3 Variant Cse4 to Centromeres and to Maintain a Functional Kinetochore",
abstract = "The kinetochore is a complex multiprotein structure located at centromeres that is essential for proper chromosome segregation. Budding-yeast Cse4 is an essential evolutionarily conserved histone H3 variant recruited to the centromere by an unknown mechanism. We have identified Scm3, an inner kinetochore protein that immunopurifies with Cse4. Scm3 is essential for viability and localizes to all centromeres. Construction of a conditional SCM3 allele reveals that depletion results in metaphase arrest, with duplicated spindle poles, short spindles, and unequal DNA distribution. The metaphase arrest is mediated by the mitotic spindle checkpoint being dependent on Mad1 and the Aurora kinase B homolog Ipl1. Scm3 interacts with both Ndc10 and Cse4 and is essential to establish centromeric chromatin after DNA replication. In addition, Scm3 is required to maintain kinetochore function throughout the cell cycle. We propose a model in which Ndc10/Scm3 binds to centromeric DNA, which is in turn essential for targeting Cse4 to centromeres.",
keywords = "DNA",
author = "Raymond Camahort and Bing Li and Laurence Florens and Swanson, {Selene K.} and Washburn, {Michael P.} and Gerton, {Jennifer L.}",
note = "Funding Information: We thank Paul Megee, Sue Biggins, Sue Jaspersen, Fred Winston, Mark Winey, and Kerry Bloom for strains and Sue J. for reagents, assistance with microscopy, and helpful discussions. We thank Josh Wunderlich, Jeff Haug, and the Stowers Institute cytometry facility for assistance with FACS analysis. We thank Arcady Mushegian for assistance with phylogenetic analysis and Chris Seidel for assistance with microarray data analysis. We thank J. Li and R. Morreale for discussions regarding centromere replication in HU-arrested cells. We thank all the members of the Gerton lab and J.L. Workman for their insightful comments on the manuscript. We extend a very appreciative thanks to Carl Wu and colleagues for communicating results prior to publication. R.C., B.L. and J.L.G. performed the experiments. R.C. and J.L.G. wrote the paper. L.F., S.K.S., and M.P.W. performed the MudPIT analysis. This research was funded by the Stowers Institute for Medical Research. ",
year = "2007",
month = jun,
day = "22",
doi = "10.1016/j.molcel.2007.05.013",
language = "English (US)",
volume = "26",
pages = "853--865",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}