Screening blockers against a potassium channel with a droplet interface bilayer array

Ruhma Syeda, Matthew A. Holden, William L. Hwang, Hagan Bayley

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

Droplet interface bilayers (DIBs) form between two lipid monolayer-encased aqueous droplets submerged in oil. Both major structural classes of membrane proteins, α-helix bundles and β barrels, represented by channels and pores, respectively, spontaneously insert into DIBs when freshly expressed by cell-free transcription and translation. Electrodes embedded within the droplets allow the measurement of transmembrane ionic currents carried by individual channels and pores. On the basis of these findings, we have devised a chip-based approach for the rapid screening of blockers against ion channels. The technique is demonstrated here with the viral potassium channel, Kcv.

Original languageEnglish (US)
Pages (from-to)15543-15548
Number of pages6
JournalJournal of the American Chemical Society
Volume130
Issue number46
DOIs
StatePublished - Nov 19 2008

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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