Screening for bladder cancer with urinary tumor markers in chemical workers with exposure to aromatic amines

Beate Pesch, Dirk Taeger, Georg Johnen, Katarzyna Gawrych, Nadine Bonberg, Christian Schwentner, Harald Wellhäußer, Matthias Kluckert, Gabriele Leng, Michael Nasterlack, Yair Lotan, Arnulf Stenzl, Thomas Brüning

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Purpose: To validate urinary markers for the early detection of bladder cancer (BC) in chemical workers.

Methods: UroScreen was conducted as a validation study for tumor markers within the frame of a health surveillance program of the German Social Accident Insurance for active or retired workers with former exposure to aromatic amines. From 2003 to 2010, 1,609 men took part in voluntary annual screens. Cytology, the quantitative NMP22® assay, and UroVysion™ were applied to 7,091 urine samples.

Results: Fifteen out of 21 tumors were detected following test positivity. The UroVysion/NMP22 panel detected 14 out of 21 tumors versus 8 tumors with cytology alone (sensitivity 66.7 vs. 44.4 %, specificity 94.5 vs. 98.5 %). The sensitivity of the panel increased to 85.7 % in samples collected ≤12 months before diagnosis and when papillomas were excluded, compared to 58.3 % with cytology. About 3 % of NMP22 tests were false-positive. UroVysion results overlapped with cytology due to the preselection of atypical cells. NMP22 was less and UroVysion more frequently positive in diluted urine samples. Leukocytes confounded NMP22 but not UroVysion. The low incidence of BC in this study population yielded low positive predictive values of the markers and high costs per tumor detected with screening.

Conclusions: UroVysion in combination with NMP22 detected more cases than cytology alone, at the expense of a lower specificity. High costs per detected case resulted from a lower BC incidence than in the past when levels of occupational exposure to aromatic amines were higher. Currently, it cannot be recommended to apply these markers for screening in asymptomatic workers. The increase in sensitivity is not balanced by the high costs of UroVysion and the false-positive tests of NMP22.

Original languageEnglish (US)
Pages (from-to)715-724
Number of pages10
JournalInternational Archives of Occupational and Environmental Health
Volume87
Issue number7
DOIs
StatePublished - Jan 1 2014

Keywords

  • Aromatic amines
  • Bladder cancer
  • Chemical workers
  • Cytology
  • NMP22
  • Screening
  • UroVysion
  • Validation study

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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    Pesch, B., Taeger, D., Johnen, G., Gawrych, K., Bonberg, N., Schwentner, C., Wellhäußer, H., Kluckert, M., Leng, G., Nasterlack, M., Lotan, Y., Stenzl, A., & Brüning, T. (2014). Screening for bladder cancer with urinary tumor markers in chemical workers with exposure to aromatic amines. International Archives of Occupational and Environmental Health, 87(7), 715-724. https://doi.org/10.1007/s00420-013-0916-3