Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: Expression of DSC2 in gastric cancer with intestinal phenotype

Katsuhiro Anami, Naohide Oue, Tsuyoshi Noguchi, Naoya Sakamoto, Kazuhiro Sentani, Tetsutaro Hayashi, Takao Hinoi, Masazumi Okajima, Jonathan M. Graff, Wataru Yasui

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Gastric cancer (GC) is one of the most common malignancies worldwide. Genes expressed only in cancer tissue, and especially on the cell membrane, will be useful molecular markers for diagnosis and may also be good therapeutic targets. To identify genes that encode transmembrane proteins present in GC, we generated Escherichia coli ampicillin secretion trap (CAST) libraries from two GC cell lines and normal stomach. By sequencing 4320 colonies from CAST libraries, we identified 30 candidate genes that encode transmembrane proteins present in GC. Quantitative reverse transcription-polymerase chain reaction analysis of these candidates revealed that ZDHHC14, BST2, DRAM2, and DSC2 were expressed much more highly in GC than in 14 kinds of normal tissues. Among these, DSC2 encodes desmocollin 2, which is one of three known desmocollins. Immunohistochemical analysis demonstrated that 22 (28%) of 80 GC cases were positive for desmocollin 2, and desmocollin 2 expression was observed frequently in GC with the intestinal mucin phenotype. Furthermore, desmocollin 2 expression was correlated with CDX2 expression. These results suggest that expression of desmocollin 2, induced by CDX2, may be a key regulator for GC with the intestinal mucin phenotype. Our results provide a list of genes that have high potential as a diagnostic and therapeutic target for GC.

Original languageEnglish (US)
Pages (from-to)275-284
Number of pages10
JournalJournal of Pathology
Volume221
Issue number3
DOIs
StatePublished - Jul 2010

Keywords

  • CAST
  • DSC2
  • Desmocollin 2
  • Gastric cancer

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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