Sebright and Campine chickens express aromatase P-450 messenger RNA inappropriately in extraglandular tissues and in skin fibroblasts

Hiroto Matsumine, Jean D. Wilson, Michael J. McPhaul

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

As the result of a single gene mutation, Sebright and Campine chickens have increased activity of cytochrome P-450 aromatase and increased formation of estrogen in extragonadal tissues. Affected roosters develop a feminizing state characterized by a feathering pattern typical of hens. In this paper we demonstrate that the expression of extraglandular aromatase in these chickens is due to the accumulation of aromatase mRNA similar to that expressed in the ovary of Leghorn and Campine hens. Furthermore, in fibroblasts cultured from Sebright skin, but not in normal Leghorn fibroblasts, aromatase mRNA levels are enhanced in response to 5-azacytidine and sodium butyrate, and aromatase mRNA levels in these fibroblasts correlate with enzymatic activity. We conclude that the accumulation of aromatase mRNA is a critical step in the expression of this mutation.

Original languageEnglish (US)
Pages (from-to)905-911
Number of pages7
JournalMolecular Endocrinology
Volume4
Issue number6
StatePublished - Jun 1990

Fingerprint

Aromatase
Chickens
Fibroblasts
Messenger RNA
Skin
Azacitidine
Mutation
Butyric Acid
Cytochrome P-450 Enzyme System
Ovary
Estrogens
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Sebright and Campine chickens express aromatase P-450 messenger RNA inappropriately in extraglandular tissues and in skin fibroblasts. / Matsumine, Hiroto; Wilson, Jean D.; McPhaul, Michael J.

In: Molecular Endocrinology, Vol. 4, No. 6, 06.1990, p. 905-911.

Research output: Contribution to journalArticle

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