Second harmonic generation imaging of corneal stroma after infection by Pseudomonas aeruginosa

Danielle M. Robertson, Nathan A. Rogers, W. Matthew Petroll, Meifang Zhu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is a pathogenic gram-negative organism that has the ability to cause blinding corneal infections following trauma and during contact lens wear. In this study, we investigated the directional movement and orientation of an invasive corneal isolate of P. aeruginosa in the corneal stroma during infection of ex vivo and in vivo rabbit corneas using multiphoton fluorescence and second harmonic generation (SHG) imaging. Ex vivo, rabbit corneas were subject to three partial thickness wounds prior to inoculation. In vivo, New Zealand white rabbits were fit with P. aeruginosa laden contact lenses in the absence of a penetrating wound. At all time points tested, infiltration of the corneal stroma by P. aeruginosa revealed a high degree of alignment between the bacteria and collagen lamellae ex vivo (p < 0.001). In vivo, P. aeruginosa traveled throughout the stroma in discrete regions or bands. Within each region, the bacteria showed good alignment with collagen lamellae (P = 0.002). Interestingly, in both the in vitro and in vivo models, P. aeruginosa did not appear to cross the corneal limbus. Taken together, our findings suggest that P. aeruginosa exploits the precise spacing of collagen lamellae in the central cornea to facilitate spread throughout the stroma.

Original languageEnglish (US)
Article number46116
JournalScientific Reports
Volume7
DOIs
StatePublished - Apr 11 2017

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Corneal Stroma
Pseudomonas aeruginosa
Infection
Cornea
Collagen
Contact Lenses
Rabbits
Limbus Corneae
Penetrating Wounds
Bacteria
Wounds and Injuries
Fluorescence

ASJC Scopus subject areas

  • General

Cite this

Second harmonic generation imaging of corneal stroma after infection by Pseudomonas aeruginosa. / Robertson, Danielle M.; Rogers, Nathan A.; Petroll, W. Matthew; Zhu, Meifang.

In: Scientific Reports, Vol. 7, 46116, 11.04.2017.

Research output: Contribution to journalArticle

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