Secreted kinase phosphorylates extracellular proteins that regulate biomineralization

Vincent S. Tagliabracci, James L. Engel, Jianzhong Wen, Sandra E. Wiley, Carolyn A. Worby, Lisa N. Kinch, Junyu Xiao, Nick V. Grishin, Jack E. Dixon

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Protein phosphorylation is a fundamental mechanism regulating nearly every aspect of cellular life. Several secreted proteins are phosphorylated, but the kinases responsible are unknown. We identified a family of atypical protein kinases that localize within the Golgi apparatus and are secreted. Fam20C appears to be the Golgi casein kinase that phosphorylates secretory pathway proteins within S-x-E motifs. Fam20C phosphorylates the caseins and several secreted proteins implicated in biomineralization, including the small integrin-binding ligand, N-linked glycoproteins (SIBLINGs). Consequently, mutations in Fam20C cause an osteosclerotic bone dysplasia in humans known as Raine syndrome. Fam20C is thus a protein kinase dedicated to the phosphorylation of extracellular proteins.

Original languageEnglish (US)
Pages (from-to)1150-1153
Number of pages4
JournalScience
Volume336
Issue number6085
DOIs
StatePublished - Jun 1 2012

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Protein Kinases
Phosphotransferases
Casein Kinases
Developmental Bone Disease
Phosphorylation
Proteins
Secretory Pathway
Protein S
Golgi Apparatus
Caseins
Integrins
Glycoproteins
Ligands
Mutation
Raine syndrome

ASJC Scopus subject areas

  • General

Cite this

Tagliabracci, V. S., Engel, J. L., Wen, J., Wiley, S. E., Worby, C. A., Kinch, L. N., ... Dixon, J. E. (2012). Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. Science, 336(6085), 1150-1153. https://doi.org/10.1126/science.1217817

Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. / Tagliabracci, Vincent S.; Engel, James L.; Wen, Jianzhong; Wiley, Sandra E.; Worby, Carolyn A.; Kinch, Lisa N.; Xiao, Junyu; Grishin, Nick V.; Dixon, Jack E.

In: Science, Vol. 336, No. 6085, 01.06.2012, p. 1150-1153.

Research output: Contribution to journalArticle

Tagliabracci, VS, Engel, JL, Wen, J, Wiley, SE, Worby, CA, Kinch, LN, Xiao, J, Grishin, NV & Dixon, JE 2012, 'Secreted kinase phosphorylates extracellular proteins that regulate biomineralization', Science, vol. 336, no. 6085, pp. 1150-1153. https://doi.org/10.1126/science.1217817
Tagliabracci, Vincent S. ; Engel, James L. ; Wen, Jianzhong ; Wiley, Sandra E. ; Worby, Carolyn A. ; Kinch, Lisa N. ; Xiao, Junyu ; Grishin, Nick V. ; Dixon, Jack E. / Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. In: Science. 2012 ; Vol. 336, No. 6085. pp. 1150-1153.
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