Secretion of prostate-specific antigen-suppressing activity by two human prostate carcinoma cell lines

Mansoor Abdul, Jer Tsong Hsieh, Christopher J. Logothetis, Naseema M. Hoosein

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The well-differentiated human prostatic carcinoma cell line LNCaP has often been used as a model to study the modulation of prostate-specific antigen (PSA) expression. In this study, we examined the effect of conditioned medium from two androgen-unresponsive, PSA-negative human prostatic carcinoma cell lines, PC-3 and DU-145, on PSA expression by the LNCaP cells. Our results show, for the first time, that the undifferentiated PC-3 and DU-145 cells secrete a factor that lowers PSA mRNA and protein levels in LNCaP cells. Further characterization of the PSA-suppressing activity indicated that it was trypsin as well as acid sensitive, heat-stable, and ammonium sulfate precipitable. The activity was present in the 30-100-kd fraction of PC-3 and DU-145 conditioned media. This PSA-suppression factor could act in an autocrine manner to render undifferentiated prostatic carcinoma cells PSA negative. It may also be responsible for the lack of increase in serum PSA levels observed in a subset of patients with hormone-resistant prostatic carcinoma.

Original languageEnglish (US)
Pages (from-to)38-41
Number of pages4
JournalUrologic Oncology: Seminars and Original Investigations
Volume1
Issue number1
DOIs
StatePublished - 1995

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Prostate-Specific Antigen
Human Activities
Prostate
Carcinoma
Cell Line
Conditioned Culture Medium
Ammonium Sulfate
Trypsin
Androgens
Hot Temperature
Hormones
Messenger RNA
Acids
Serum
Proteins

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Secretion of prostate-specific antigen-suppressing activity by two human prostate carcinoma cell lines. / Abdul, Mansoor; Hsieh, Jer Tsong; Logothetis, Christopher J.; Hoosein, Naseema M.

In: Urologic Oncology: Seminars and Original Investigations, Vol. 1, No. 1, 1995, p. 38-41.

Research output: Contribution to journalArticle

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abstract = "The well-differentiated human prostatic carcinoma cell line LNCaP has often been used as a model to study the modulation of prostate-specific antigen (PSA) expression. In this study, we examined the effect of conditioned medium from two androgen-unresponsive, PSA-negative human prostatic carcinoma cell lines, PC-3 and DU-145, on PSA expression by the LNCaP cells. Our results show, for the first time, that the undifferentiated PC-3 and DU-145 cells secrete a factor that lowers PSA mRNA and protein levels in LNCaP cells. Further characterization of the PSA-suppressing activity indicated that it was trypsin as well as acid sensitive, heat-stable, and ammonium sulfate precipitable. The activity was present in the 30-100-kd fraction of PC-3 and DU-145 conditioned media. This PSA-suppression factor could act in an autocrine manner to render undifferentiated prostatic carcinoma cells PSA negative. It may also be responsible for the lack of increase in serum PSA levels observed in a subset of patients with hormone-resistant prostatic carcinoma.",
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