Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles

M. A. Nesmeyanova, I. M. Tsfasman, A. L. Karamyshev, N. E. Suzina

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Various Escherichia coli strains were transformed by multicopy plasmids pHI-1, pHI-7 and pPHOI carrying the entire regulatory and structural phoA sequences. All transformants with the intact pho regulatory system displayed PhoA oversynthesis and secretion into the medium. They also accumulated the alkaline phosphatase precursor localized in the outer membrane fraction. The dynamics of enzyme synthesis and secretion as well as cell cytomorphology during secretion were studied in strain E. coli K12 802 carrying pHI-7 plasmid. PhoA protein was shown to be selectively released into the medium in vesicles budding from outer membrane.

Original languageEnglish (US)
Pages (from-to)394-406
Number of pages13
JournalWorld Journal of Microbiology & Biotechnology
Volume7
Issue number3
DOIs
StatePublished - May 1 1991

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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