Secretome analysis of novel IgE-binding proteins from Penicillium citrinum

Li Li Chiu, Kuang Lun Lee, Yu Fen Lin, Chia Yu Chu, Son Nan Su, Lu Ping Chow

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Penicillium genus of fungi is a frequently reported cause of allergic reactions. However, only a limited number of allergens have been reported. In Penicillium spp., many allergens show higher IgE-binding activity in culture filtrate extracts than in cellular extracts. In order to investigate the IgE-reactive profile of mold-sensitized patients, secreted IgE-reactive proteins from Penicillium citrinum were identified by 2-DE, serum immunoblotting, and nanoLC-MS/MS. Among the IgE-reactive spots, one known allergen, Pen c 13, and four novel allergens were identified. The cDNAs coding for Pen c 32 and Pen c 30 were cloned using designed primers based on nanoLC-MS/MS analysis. The amino acid sequences of Pen c 32 and Pen c 30 were, respectively, found to have extensive similarity with those of pectate lyases and catalases from various fungi. Native Pen c 30 was shown to have catalase activity and to bind to serum IgE from 48% of mold-allergic patients and induced immediate type skin reactions in a sensitized patient Here, we present a proteome approach which resulted in the identification of four novel secreted allergens. These novel allergens might be useful in allergy diagnosis and in the treatment of mold-allergic disorders.

Original languageEnglish (US)
Pages (from-to)33-45
Number of pages13
JournalProteomics - Clinical Applications
Volume2
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Galectin 3
Penicillium
Allergens
Immunoglobulin E
Fungi
Catalase
Hypersensitivity
Allergies
Proteome
Serum
Immunoblotting
Amino Acid Sequence
Skin
Complementary DNA
Amino Acids
Proteins

Keywords

  • Immunoglobulin E-reactive protein
  • Pen c 30
  • Pen c 32
  • Penicillium citrinum
  • Secretome

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Chiu, L. L., Lee, K. L., Lin, Y. F., Chu, C. Y., Su, S. N., & Chow, L. P. (2008). Secretome analysis of novel IgE-binding proteins from Penicillium citrinum. Proteomics - Clinical Applications, 2(1), 33-45. https://doi.org/10.1002/prca.200780050

Secretome analysis of novel IgE-binding proteins from Penicillium citrinum. / Chiu, Li Li; Lee, Kuang Lun; Lin, Yu Fen; Chu, Chia Yu; Su, Son Nan; Chow, Lu Ping.

In: Proteomics - Clinical Applications, Vol. 2, No. 1, 01.2008, p. 33-45.

Research output: Contribution to journalArticle

Chiu, Li Li ; Lee, Kuang Lun ; Lin, Yu Fen ; Chu, Chia Yu ; Su, Son Nan ; Chow, Lu Ping. / Secretome analysis of novel IgE-binding proteins from Penicillium citrinum. In: Proteomics - Clinical Applications. 2008 ; Vol. 2, No. 1. pp. 33-45.
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