Abstract
We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 Å resolution. α helices are clearly visible and β sheet density is also visible at this resolution. While the overall conformation of this structure is quite consistent with the published X-ray data, a measurable shift in the positions of three α helices in the intermediate domain is observed, not consistent with any of the 7 monomeric structures in the Protein Data Bank model (1OEL). In addition, there is evidence for slight rearrangement or flexibility in parts of the apical domain. The 6 Å resolution cryo-EM GroEL structure clearly demonstrates the veracity and expanding scope of cryo-EM and the single particle reconstruction technique for macromolecular machines.
Original language | English (US) |
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Pages (from-to) | 1129-1136 |
Number of pages | 8 |
Journal | Structure |
Volume | 12 |
Issue number | 7 |
DOIs |
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State | Published - Jul 2004 |
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology