Seeing GroEL at 6 Å resolution by single particle electron cryomicroscopy

Steven J. Ludtke, Dong Hua Chen, Jiu Li Song, David T. Chuang, Wah Chiu

Research output: Contribution to journalComment/debate

161 Scopus citations

Abstract

We present a reconstruction of native GroEL by electron cryomicroscopy (cryo-EM) and single particle analysis at 6 Å resolution. α helices are clearly visible and β sheet density is also visible at this resolution. While the overall conformation of this structure is quite consistent with the published X-ray data, a measurable shift in the positions of three α helices in the intermediate domain is observed, not consistent with any of the 7 monomeric structures in the Protein Data Bank model (1OEL). In addition, there is evidence for slight rearrangement or flexibility in parts of the apical domain. The 6 Å resolution cryo-EM GroEL structure clearly demonstrates the veracity and expanding scope of cryo-EM and the single particle reconstruction technique for macromolecular machines.

Original languageEnglish (US)
Pages (from-to)1129-1136
Number of pages8
JournalStructure
Volume12
Issue number7
DOIs
StatePublished - Jul 2004

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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