TY - JOUR
T1 - Seipin is required for converting nascent to mature lipid droplets
AU - Wang, Huajin
AU - Becuwe, Michel
AU - Housden, Benjamin E.
AU - Chitraju, Chandramohan
AU - Porras, Ashley J.
AU - Graham, Morven M.
AU - Liu, Xinran N.
AU - Thiam, Abdou Rachid
AU - Savage, David B.
AU - Agarwal, Anil K.
AU - Garg, Abhimanyu
AU - Olarte, Maria Jesus
AU - Lin, Qingqing
AU - Frohlich, Florian
AU - Hannibal-Bach, Hans Kristian
AU - Upadhyayula, Srigokul
AU - Perrimon, Norbert
AU - Kirchhausen, Tomas
AU - Ejsing, Christer S.
AU - Walther, Tobias C.
AU - Farese, Robert V.
N1 - Publisher Copyright:
© Wang et al.
PY - 2016/8/26
Y1 - 2016/8/26
N2 - How proteins control the biogenesis of cellular lipid droplets (LDs) is poorly understood. Using Drosophila and human cells, we show here that seipin, an ER protein implicated in LD biology, mediates a discrete step in LD formation—the conversion of small, nascent LDs to larger, mature LDs. Seipin forms discrete and dynamic foci in the ER that interact with nascent LDs to enable their growth. In the absence of seipin, numerous small, nascent LDs accumulate near the ER and most often fail to grow. Those that do grow prematurely acquire lipid synthesis enzymes and undergo expansion, eventually leading to the giant LDs characteristic of seipin deficiency. Our studies identify a discrete step of LD formation, namely the conversion of nascent LDs to mature LDs, and define a molecular role for seipin in this process, most likely by acting at ER-LD contact sites to enable lipid transfer to nascent LDs.
AB - How proteins control the biogenesis of cellular lipid droplets (LDs) is poorly understood. Using Drosophila and human cells, we show here that seipin, an ER protein implicated in LD biology, mediates a discrete step in LD formation—the conversion of small, nascent LDs to larger, mature LDs. Seipin forms discrete and dynamic foci in the ER that interact with nascent LDs to enable their growth. In the absence of seipin, numerous small, nascent LDs accumulate near the ER and most often fail to grow. Those that do grow prematurely acquire lipid synthesis enzymes and undergo expansion, eventually leading to the giant LDs characteristic of seipin deficiency. Our studies identify a discrete step of LD formation, namely the conversion of nascent LDs to mature LDs, and define a molecular role for seipin in this process, most likely by acting at ER-LD contact sites to enable lipid transfer to nascent LDs.
UR - http://www.scopus.com/inward/record.url?scp=84988807217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988807217&partnerID=8YFLogxK
U2 - 10.7554/eLife.16582
DO - 10.7554/eLife.16582
M3 - Article
C2 - 27564575
AN - SCOPUS:84988807217
SN - 2050-084X
VL - 5
JO - eLife
JF - eLife
IS - AUGUST
M1 - e16582
ER -