Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

A. Frankel, D. Schlossman, P. Welsh, A. Hertler, D. Withers, S. Johnston

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving residues located in the proposed active-site cleft.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalMolecular and Cellular Biology
Volume9
Issue number2
StatePublished - Feb 1989

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Ricinus
Ricin
Saccharomyces cerevisiae
Mutation
Catalytic Domain
Alleles
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Frankel, A., Schlossman, D., Welsh, P., Hertler, A., Withers, D., & Johnston, S. (1989). Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae. Molecular and Cellular Biology, 9(2), 415-420.

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae. / Frankel, A.; Schlossman, D.; Welsh, P.; Hertler, A.; Withers, D.; Johnston, S.

In: Molecular and Cellular Biology, Vol. 9, No. 2, 02.1989, p. 415-420.

Research output: Contribution to journalArticle

Frankel, A, Schlossman, D, Welsh, P, Hertler, A, Withers, D & Johnston, S 1989, 'Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.', Molecular and Cellular Biology, vol. 9, no. 2, pp. 415-420.
Frankel, A. ; Schlossman, D. ; Welsh, P. ; Hertler, A. ; Withers, D. ; Johnston, S. / Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae. In: Molecular and Cellular Biology. 1989 ; Vol. 9, No. 2. pp. 415-420.
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