Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin

F. Stirpe; E. J. Wawrzynczak; A. N F Brown; R. E. Knyba; G. J. Watson; L. Barbieri; P. E. Thorpe

doi:10.1038/bjc.1988.258

Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin

F. Stirpe, E. J. Wawrzynczak, A. N F Brown, R. E. Knyba, G. J. Watson, L. Barbieri, P. E. Thorpe

Pharmacology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell* line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKRA cells by 50% at a concentration of 1-4 x 10^‑11 M as compared with 1 x 10^‑9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10^‑8 M. Further, bryodin and momordin immunotoxins made from an antibody (RIO) of irrelevant specificity were without effect on AKR-A cells.

Original language	English (US)
Pages (from-to)	558-561
Number of pages	4
Journal	British journal of cancer
Volume	58
Issue number	5
DOIs	https://doi.org/10.1038/bjc.1988.258
State	Published - Nov 1988

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1038/bjc.1988.258

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title = "Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin",

abstract = "The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell* line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKRA cells by 50% at a concentration of 1-4 x 10‑11 M as compared with 1 x 10‑9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10‑8 M. Further, bryodin and momordin immunotoxins made from an antibody (RIO) of irrelevant specificity were without effect on AKR-A cells.",

author = "F. Stirpe and Wawrzynczak, {E. J.} and Brown, {A. N F} and Knyba, {R. E.} and Watson, {G. J.} and L. Barbieri and Thorpe, {P. E.}",

note = "Funding Information: The work performed in Bologna was supported by a contract from the Consiglio Nazionale delle Ricerche, Rome, by grants from the Associazione Italiana per la Ricerca sul Cancro, Milan, and from the Ministero della Pubblica Istruzione, Rome and by the Pallotti's Legacy for Cancer Research. typing the manuscript. We thank Mrs A. Becket for",

year = "1988",

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doi = "10.1038/bjc.1988.258",

language = "English (US)",

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pages = "558--561",

journal = "British journal of cancer",

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AU - Stirpe, F.

AU - Wawrzynczak, E. J.

AU - Brown, A. N F

AU - Knyba, R. E.

AU - Watson, G. J.

AU - Barbieri, L.

AU - Thorpe, P. E.

N1 - Funding Information: The work performed in Bologna was supported by a contract from the Consiglio Nazionale delle Ricerche, Rome, by grants from the Associazione Italiana per la Ricerca sul Cancro, Milan, and from the Ministero della Pubblica Istruzione, Rome and by the Pallotti's Legacy for Cancer Research. typing the manuscript. We thank Mrs A. Becket for

PY - 1988/11

Y1 - 1988/11

N2 - The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell* line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKRA cells by 50% at a concentration of 1-4 x 10‑11 M as compared with 1 x 10‑9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10‑8 M. Further, bryodin and momordin immunotoxins made from an antibody (RIO) of irrelevant specificity were without effect on AKR-A cells.

AB - The ribosome-inactivating proteins, bryodin, from Bryonia dioica, and momordin, from Momordica charantia, were coupled by a disulphide bond to a monoclonal anti-Thy 1.1 antibody (OX7). Both immunotoxins were specifically cytotoxic to the Thy 1.1-expressing mouse lymphoma cell* line AKR-A in vitro. The OX7-bryodin immunotoxins were the more powerfully toxic and reduced protein synthesis in AKRA cells by 50% at a concentration of 1-4 x 10‑11 M as compared with 1 x 10‑9 M for the OX7-momordin immunotoxins. Neither of the immunotoxins was toxic to mouse lymphoma EL4 cells, which lack the Thy 1.1 antigen, at concentrations up to 3 x 10‑8 M. Further, bryodin and momordin immunotoxins made from an antibody (RIO) of irrelevant specificity were without effect on AKR-A cells.

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Selective cytotoxic activity of immunotoxins composed of a monoclonal anti-thy 1.1 antibody and the ribosome-inactivating proteins bryodin and momordin

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