TY - JOUR
T1 - Selective expression of the Ly-6C.2 epitope on Lyt-2+ T cells that can develop in the absence of thymic influence
AU - Leo, O.
AU - Foo, M.
AU - Forman, J.
AU - Shivakumar, S.
AU - Rabinowitz, R.
AU - Bluestone, J. A.
PY - 1988
Y1 - 1988
N2 - The present study focused on attempts to correlate the expression of T cell differentiation antigens and development. A series of mAb was developed that recognized an Ly-6.2-linked Ag, Ly-6C.2. These mAb reacted with a subset of peripheral Lyt-2+ T lymphocytes but not Lyt-2+ thymocytes. Functional analysis of the Lyt-2+,Ly-6C.2- and Lyt-2+,Ly-6C.2+ subsets demonstrated that both cell types responeded in class I and class II allogeneic MLC as well as in virus-specific cell-mediated cytolysis assay, although precursor frequency analysis suggested that the majority of precursor CTL were Ly-6C.2+. The marker did not appear to represent an activation Ag because Ly-6C.2-, Lyt-2+ precursor CTl generated Ly-6C.2-, Lyt-2+ CTL. Finally, virtually all splenic Lyt-2+ T cells from athymic (nu/nu) mice were Ly-6C.2+ whereas all thymic CTL were Ly-6C.2-. These results suggested that Ly-6C.2+,Lyt-2+ T cells may represent a distinct lineage of Lyt-2+ cells that can develop in the absence of thymic influence.
AB - The present study focused on attempts to correlate the expression of T cell differentiation antigens and development. A series of mAb was developed that recognized an Ly-6.2-linked Ag, Ly-6C.2. These mAb reacted with a subset of peripheral Lyt-2+ T lymphocytes but not Lyt-2+ thymocytes. Functional analysis of the Lyt-2+,Ly-6C.2- and Lyt-2+,Ly-6C.2+ subsets demonstrated that both cell types responeded in class I and class II allogeneic MLC as well as in virus-specific cell-mediated cytolysis assay, although precursor frequency analysis suggested that the majority of precursor CTL were Ly-6C.2+. The marker did not appear to represent an activation Ag because Ly-6C.2-, Lyt-2+ precursor CTl generated Ly-6C.2-, Lyt-2+ CTL. Finally, virtually all splenic Lyt-2+ T cells from athymic (nu/nu) mice were Ly-6C.2+ whereas all thymic CTL were Ly-6C.2-. These results suggested that Ly-6C.2+,Lyt-2+ T cells may represent a distinct lineage of Lyt-2+ cells that can develop in the absence of thymic influence.
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M3 - Article
C2 - 2454263
AN - SCOPUS:0023801650
SN - 0022-1767
VL - 141
SP - 37
EP - 44
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -